Ogawa K, Narasaki M, Mori M
Toxicol Pathol. 1987;15(1):69-72. doi: 10.1177/019262338701500108.
Heterogenous rabbit antisera were prepared against microsomal proteins of hyperplastic hepatic nodules (HPN) induced by chemicals, and were utilized to assess the antigenic differences of microsomal polypeptides within a normal liver, HPN, and hepatocellular carcinomas (HCC), utilizing immunodetection of antigens separated electrophoretically and transferred to nitrocellulose. Although most antigens were common to all microsomes, differences (increase or decrease) were noted in some polypeptides not only between the normal liver and HPN, but also between HPN and HCC. On the other hand, monoclonal antibodies against epoxide hydrolase (EH), which was initially found as the PN antigen, reacted to a single polypeptide with a molecular weight of 49,000 in all the microsomes. These results suggested that there is little molecular modification of EH during hepatic carcinogenesis.
制备了针对化学诱导的增生性肝结节（HPN）微粒体蛋白的异种兔抗血清，并利用免疫检测法评估正常肝脏、HPN和肝细胞癌（HCC）中微粒体多肽的抗原差异，该免疫检测法用于检测经电泳分离并转移至硝酸纤维素膜上的抗原。尽管大多数抗原在所有微粒体中都很常见，但不仅在正常肝脏和HPN之间，而且在HPN和HCC之间，一些多肽存在差异（增加或减少）。另一方面，最初作为PN抗原被发现的针对环氧化物水解酶（EH）的单克隆抗体，在所有微粒体中都与一条分子量为49000的单一多肽发生反应。这些结果表明，在肝癌发生过程中EH的分子修饰很少。
