Department of Chemistry, University of Hong Kong, Hong Kong, SAR, China.
Methods Mol Biol. 2022;2530:33-43. doi: 10.1007/978-1-0716-2489-0_3.
Serine/threonine ligation (STL) and cysteine/penicillamine ligation (CPL) are highly chemo- and regioselective reactions between unprotected peptides with C-terminus salicylaldehyde esters and unprotected peptides with N-terminus serine/threonine or cysteine/penicillamine, which serve as powerful tools for cyclic peptide natural product and chemical protein synthesis. Herein, we introduce the preparation of C-terminal peptide salicylaldehyde esters, serine/threonine ligation, cysteine/penicillamine ligation, and subsequent acidolysis.
丝氨酸/苏氨酸连接（STL）和半胱氨酸/青霉胺连接（CPL）是未保护的 C 末端水杨醛酯肽与未保护的 N 末端丝氨酸/苏氨酸或半胱氨酸/青霉胺之间的高度化学和区域选择性反应，它们是环状肽天然产物和化学蛋白质合成的有力工具。在此，我们介绍了 C 末端肽水杨醛酯的制备、丝氨酸/苏氨酸连接、半胱氨酸/青霉胺连接以及随后的酸解。
