鸭甲型肝炎病毒 1 型介导 S 期细胞周期停滞。

Duck hepatitis A virus type 1 mediates cell cycle arrest in the S phase.

机构信息

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, 611130, Sichuan, China.

Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu City, 611130, Sichuan, China.

出版信息

Virol J. 2022 Jun 27;19(1):111. doi: 10.1186/s12985-022-01839-6.

DOI:10.1186/s12985-022-01839-6

PMID:35761382

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9235186/

Abstract

BACKGROUND

Duck hepatitis A virus type 1 (DHAV-1) is one of the most serious pathogens endangering the duck industry. However, there are few studies on the regulation of the cell cycle by DHAV-1.

METHODS

In this study, flow cytometry was applied to analyze the effect of DHAV-1 infection on the cell cycle of duck embryo fibroblasts (DEFs). Subsequently, we analyzed the effects of cell cycle phases on DHAV-1 replication by real-time reverse transcriptase quantitative PCR (real-time RT-qPCR).

RESULTS

Flow cytometry data analysis found that DEFs in the S phase increased by 25.85% and 54.21% at 24 h and 48 h after DHAV-1 infection, respectively. The levels of viral RNA detected by real-time RT-qPCR were higher in the DEFs with synchronization in the S phase or G0/G1 phase than in the control group. However, there was no difference in viral copy number between the G2/M phase arrest and control groups. In addition, non-structural protein 3D of DHAV-1 significantly increased cells in the S phase, indicating that 3D protein is one of the reasons for the cell cycle arrest in the S phase.

CONCLUSIONS

In summary, DHAV-1 infection induces the cell cycle arrest of DEFs in the S phase. Both S phase and G0/G1 phase synchronization facilitate the replication of DHAV-1, and 3D protein is one of the reasons for the S phase arrest.

摘要

背景

鸭甲型肝炎病毒 1 型（DHAV-1）是危害养鸭业的最严重病原体之一。然而，关于 DHAV-1 对细胞周期的调控作用的研究较少。

方法

本研究采用流式细胞术分析 DHAV-1 感染对鸭胚成纤维细胞（DEFs）细胞周期的影响。随后，我们通过实时逆转录定量 PCR（real-time RT-qPCR）分析了细胞周期各时相对 DHAV-1 复制的影响。

结果

流式细胞术数据分析发现，DHAV-1 感染后 24 和 48 h，DEFs 处于 S 期的比例分别增加了 25.85%和 54.21%。实时 RT-qPCR 检测到的病毒 RNA 水平在 S 期或 G0/G1 期同步的 DEF 中高于对照组。然而，G2/M 期阻滞与对照组之间的病毒拷贝数没有差异。此外，DHAV-1 的非结构蛋白 3D 显著增加了 S 期细胞，表明 3D 蛋白是 S 期细胞周期阻滞的原因之一。