MOE Key Laboratory of New Processing Technology for Nonferrous Metals and Materials, Guangxi Key Laboratory of Processing for Non-ferrous Metals and Featured Materials, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
Institute of Chemical Sciences, University of Peshawar, 25120 KP, Pakistan.
Food Res Int. 2022 Jul;157:111406. doi: 10.1016/j.foodres.2022.111406. Epub 2022 May 26.
Antioxidant peptides from edible protein sources have attracted great attention due to their high activity, easy adsorption and low toxicity. The purpose of this study was to develop a new MOF material with specific recognition and capture ability to achieve the aim of highly efficient and selective antioxidant peptides from silkworm pupa protein hydrolysate-N (SPPH-N). Firstly, Histidine (His) widely presented in antioxidant peptides was used to in-suit construct the modified ZIF-8 material (ZIF-His) with imprinted defective mesopores, based on "molecular imprinting strategy". Then, it was applied to extract antioxidant peptides from SPPH-N. ZIF-His exhibited high adsorption uptake for antioxidant peptides and especially presented specific adsorption property for peptides contained His, tryptophan, phenylalanine and tyrosine. Three peptides with the highest antioxidant activity (FKVPNMY, AVNMVPFPR and VNMVPFPR) from ZIF-His eluent were identified by Nano-LC-MS/MS. These peptides were synthesized and presented the excellent free radicals scavenging activity and the Ferric reducing power.
由于其高活性、易吸收和低毒性，来源于食用蛋白的抗氧化肽引起了广泛关注。本研究旨在开发一种具有特定识别和捕获能力的新型 MOF 材料，以实现从蚕蛹蛋白水解物-N（SPPH-N）中高效、选择性地提取抗氧化肽的目的。首先，基于“分子印迹策略”，利用抗氧化肽中广泛存在的组氨酸（His）原位构建具有印迹缺陷介孔的修饰 ZIF-8 材料（ZIF-His）。然后，将其应用于从 SPPH-N 中提取抗氧化肽。ZIF-His 对抗氧化肽具有较高的吸附摄取能力，特别是对含有 His、色氨酸、苯丙氨酸和酪氨酸的肽具有特异性吸附性能。通过 Nano-LC-MS/MS 从 ZIF-His 洗脱液中鉴定出三种具有最高抗氧化活性的肽（FKVPNMY、AVNMVPFPR 和 VNMVPFPR）。这些肽被合成并表现出优异的自由基清除活性和铁还原能力。
