School of Agricultural Engineering and Food Science, Shandong University of Technology, Zi'bo 255049, People's Republic of China.
School of Agricultural Engineering and Food Science, Shandong University of Technology, Zi'bo 255049, People's Republic of China.
Food Res Int. 2022 Jul;157:111418. doi: 10.1016/j.foodres.2022.111418. Epub 2022 May 27.
To better understand the global changes of amino acid catabolism and anabolism in broccoli in response to high O stress, iTRAQ-based proteomics combined with amino acid analysis was used to investigate the broccoli proteome at 0 and 4 d after treatment with different O concentrations (5% O + 5% CO, 20% O + 5% CO and 40% O + 5% CO) at 20℃. A total of 106 proteins with changes ≥ 1.2-fold in abundance were observed. Amino acid anabolism was significantly suppressed by high O stress, while catabolism was enhanced. High O stress-induced amino acid metabolism promoted the conversion of Met to ethylene and the degradation of amino acids to intermediate metabolites of the TCA cycle, thereby suppressing glucosinolate biosynthesis. However, the up-regulation of arginase and urease induced by high O stress aggravated ammonium toxicity. These findings enhance our understanding of high O stress-induced amino acid metabolism, as well as the effects of amino acid metabolism on broccoli senescence.
为了更好地了解 broccoli 中氨基酸分解代谢和合成代谢对高 O 胁迫的全球变化，本研究采用 iTRAQ 基于蛋白质组学联合氨基酸分析的方法，研究了在 20℃下用不同 O 浓度（5% O+5% CO、20% O+5% CO 和 40% O+5% CO）处理 0 和 4 d 后 broccoli 的蛋白质组。共观察到 106 种丰度变化≥1.2 倍的蛋白质。高 O 胁迫显著抑制氨基酸合成代谢，而促进分解代谢。高 O 胁迫诱导的氨基酸代谢促进 Met 向乙烯的转化和氨基酸降解为 TCA 循环中间代谢物，从而抑制了硫代葡萄糖苷的生物合成。然而，高 O 胁迫诱导的精氨酸酶和脲酶的上调加剧了铵毒性。这些发现增进了我们对高 O 胁迫诱导的氨基酸代谢以及氨基酸代谢对 broccoli 衰老的影响的理解。
