Food Technology Division, ceiA3, CIAMBITAL, University of Almería, 04120 Almería, Spain.
Chemical Engineering Department, University of Almería, 04120 Almería, Spain.
Food Res Int. 2022 Jul;157:111421. doi: 10.1016/j.foodres.2022.111421. Epub 2022 May 28.
Ahiflower oil© is extracted from the seeds of Buglossoides arvensis, which contains high amounts of stearidonic acid (SDA, 18:4n-3), while its phenolic composition still is unreported. Moreover, several Buglossoides taxa remain unstudied and could become natural sources of SDA. In this work, seeds of several Buglossoides taxa and Ahiflower oil© were screened for fatty acids, phenolic compounds, and in vitro antiproliferative activities against colorectal cancer cells. Four flavonoids and 16 phenolic acids were identified and quantified. Among Buglossoides taxa, the highest amounts of phenolic compounds were found in samples collected in Spain, under a warm Mediterranean climate. Rosmarinic and lithospermic acids were the main phenols found in Buglossoides seeds. The MTT assay showed dose- and time-dependent inhibitory effects of B. arvensis extracts on HT-29 cancer cells, with a GIvalue of ∼280 μg/mL after 72 h of cell exposure to seed extracts. The latter showed lower antiproliferative activity than that of pure phenolics due to the simultaneous presence of other compounds in the extracts, as evidenced by H NMR. This work constitutes the first approach to evaluate the seeds of several Buglossoides taxa as functional oils-providers to use them as functional foods.
芝麻菜籽油取自芝麻菜的种子，含有大量的 stearidonic 酸（SDA，18:4n-3），但其酚类成分尚未报道。此外，一些芝麻菜属的分类群仍未被研究，可能成为 SDA 的天然来源。在这项工作中，对几种芝麻菜属植物的种子和芝麻菜籽油进行了脂肪酸、酚类化合物和体外抗结肠直肠癌细胞增殖活性的筛选。鉴定并定量了 4 种类黄酮和 16 种酚酸。在芝麻菜属植物中，在温暖的地中海气候下，西班牙采集的样本中酚类化合物含量最高。迷迭香酸和丹参酸是芝麻菜种子中主要的酚类物质。MTT 试验显示，B. arvensis 提取物对 HT-29 癌细胞具有剂量和时间依赖性的抑制作用，在细胞暴露于种子提取物 72 小时后，GI 值约为 280μg/mL。由于提取物中同时存在其他化合物，后者的抗增殖活性低于纯酚类物质，这一点可以通过 H NMR 得到证明。这项工作首次评估了几种芝麻菜属植物的种子作为功能性油的提供者，以将它们用作功能性食品。
