Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Laboratory of Signal Transduction, Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
Department of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Immunomodulation of Natural Products Research Group, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Food Res Int. 2022 Jul;157:111433. doi: 10.1016/j.foodres.2022.111433. Epub 2022 May 28.
Bisphenol A (BPA) has been reported to have neurotoxic properties that may increase the risk of neurodegenerative diseases by inducing neuroinflammation. Auricularia polytricha (AP) is an edible mushroom with several medicinal properties. Herein, the anti-neuroinflammatory effects of AP extracts against BPA-induced inflammation of BV2 microglial cells were investigated. Hexane (APH) and ethanol (APE) extracts of AP inhibited BPA-induced neuroinflammation in BV2 microglia by reducing microglial activation and the expression of pro-inflammatory cytokines. These anti-inflammatory effects were regulated by the NF-κB signaling pathway. In addition, APH and APE exhibited antioxidative effects by increasing the activity of the SOD-1 enzyme and restoring the accumulation of reactive oxygen species (ROS) in BPA-induced BV2 cells. Moreover, the conditioned medium prepared using BPA-induced BV2 cells demonstrated that the presence of APH or APE could attenuate ROS production in HT-22 cells. Further, ergosterol was isolated from APE and also showed anti-inflammatory and antioxidative activities. In conclusion, AP extracts and ergosterol attenuated neuroinflammation against BPA induction in BV2 microglial cells through the NF-κB signaling pathway.
双酚 A（BPA）已被报道具有神经毒性，通过诱导神经炎症可能会增加神经退行性疾病的风险。银耳（AP）是一种具有多种药用特性的食用蘑菇。在此，研究了银耳提取物对 BPA 诱导的 BV2 小胶质细胞炎症的抗炎作用。AP 的正己烷（APH）和乙醇（APE）提取物通过减少小胶质细胞活化和促炎细胞因子的表达来抑制 BV2 小胶质细胞中的 BPA 诱导的神经炎症。这些抗炎作用受 NF-κB 信号通路调节。此外，APH 和 APE 通过增加 SOD-1 酶的活性并恢复 BPA 诱导的 BV2 细胞中活性氧（ROS）的积累来表现出抗氧化作用。此外，使用 BPA 诱导的 BV2 细胞制备的条件培养基表明，APH 或 APE 的存在可以减轻 HT-22 细胞中 ROS 的产生。此外，从 APE 中分离出麦角固醇，也表现出抗炎和抗氧化活性。总之，银耳提取物和麦角固醇通过 NF-κB 信号通路减轻了 BV2 小胶质细胞中 BPA 诱导的神经炎症。
