Department of Biology, Faculty of Science, University of Hradec Kralove, Rokitanskeho 62, CZ-50003 Hradec Kralove, Czech Republic.
Department of Biology, Faculty of Science, University of Hradec Kralove, Rokitanskeho 62, CZ-50003 Hradec Kralove, Czech Republic.
Food Res Int. 2022 Jul;157:111473. doi: 10.1016/j.foodres.2022.111473. Epub 2022 Jun 7.
Blood sausages consisting of groats, pork, porcine offal, fat, blood, and spices are very popular in the Czech Republic. All these ingredients are potential sources of dietary exposure to ochratoxin A (OTA). OTA has a strong affinity to serum proteins in porcine blood. Thus, the contamination of blood sausages with OTA can be expected. This study aims to evaluate OTA in 200 samples of porcine blood sausages purchased at the Czech market during 2020-2021. The analytical method high-performance liquid chromatography coupled with fluorescence detection with pre-treatment using immunoaffinity columns was employed to determine OTA. The limit of detection was 0.03 ng/g and the limit of quantification 0.10 ng/g. Recovery was 71.6 %. All samples were positive at contents ranging from 0.15 to 5.68 ng/g with a mean of 1.47 ng/g, and a median of 1.26 ng/g. A total of 66% of these samples contained OTA content exceeding the maximum limit of 1 ng/g set in Italy. This study demonstrates that the Czech population is exposed to OTA from blood sausages. The proposed preliminary action limit for OTA in blood sausages should be set at 1 ng/g. No regulatory limits for OTA in blood sausages have been established yet in the European Union legislation. To protect human health, further monitoring of OTA in these products is necessary.
血肠由燕麦、猪肉、猪内脏、脂肪、血液和香料制成，在捷克共和国非常受欢迎。所有这些成分都是膳食中接触赭曲霉毒素 A（OTA）的潜在来源。OTA 与猪血液中的血清蛋白具有很强的亲和力。因此，可以预期血肠会受到 OTA 的污染。本研究旨在评估 2020-2021 年期间在捷克市场购买的 200 份猪血肠中的 OTA。采用高效液相色谱-荧光检测法，用免疫亲和柱进行前处理，分析方法用于测定 OTA。检测限为 0.03ng/g，定量限为 0.10ng/g。回收率为 71.6%。所有样品均呈阳性，含量范围为 0.15-5.68ng/g，平均值为 1.47ng/g，中位数为 1.26ng/g。这些样品中共有 66%的样品含有超过意大利设定的 1ng/g 最大限量的 OTA。本研究表明，捷克人群正从血肠中接触到 OTA。应将血肠中 OTA 的初步行动限量设定为 1ng/g。欧盟法规尚未为血肠中的 OTA 设定监管限量。为了保护人类健康，有必要对这些产品中的 OTA 进行进一步监测。
