Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233000 Anhui, China.
Department of Emergency Internal Medicine, The Third the People's Hospital of Bengbu, Bengbu, 233000 Anhui, China.
Comput Math Methods Med. 2022 Jun 18;2022:3516335. doi: 10.1155/2022/3516335. eCollection 2022.
Chitinase-3-like protein 1 (CHI3Ll) has been identified as a novel tumor marker in several cancers. The objective of this study was to detect the expression of Chitinase-3-like protein 1 (CHI3L1) and CD31-labeled microvessel density (MVD) in cervical squamous cell carcinoma (CSCC) and to assess its prognostic impact.
Elivision™ plus immunohistochemical method was used to detect CHI3L1 expression and MVD in different cervical tissues. We analyzed the relationship between CHI3L1 and MVD in CSCC tissues and investigated the relationship between CHI3L1, MVD, and clinicopathological parameters. Univariate and multivariate survival analyses were performed to assess the impact on progression-free survival (PFS) and overall survival (OS).
The positive expression rate of CHI3L1 protein in CSCC tissues (69.9%, 72/103) was significantly higher than that in high-grade cervical intraepithelial lesions (53.3%, 32/60), low-grade cervical intraepithelial lesions (25%, 15/60), and normal cervical tissues (16.7%, 10/60). MVD values ranged from 6 to 64 in CSCC, and no microvascular formation was observed in normal cervical tissues, high-grade intraepithelial lesions, or low-grade intraepithelial lesions. The high expression of CHI3L1 and MVD was significantly correlated with the invasion depth, differentiation degree, vascular invasion, and lymph node metastasis of CSCC (all < 0.05). In CSCC, the expression of MVD in the CHI3L1 high-expression group (41.35 ± 9.056) was significantly higher than that in the CHI3L1 low-expression group (23.26 ± 11.000, < 0.05). On univariate Kaplan-Meier analysis, FIGO stage, tumor diameter, lymph node metastasis, vascular invasion, CHI3L1, and MVD of CSCC were related to the prognosis of PFS and OS (all < 0.05); however, CHI3L1 and MVD were not independent prognostic factors.
CHI3L1 may be involved in the progression of cervical cancer. Its high expression can promote neovascularization in the tumor microenvironment. CHI3L1 is a potential therapeutic target in the context of cervical cancer.
壳三糖酶蛋白 3 样蛋白 1（CHI3Ll）已被确定为几种癌症中的一种新型肿瘤标志物。本研究的目的是检测壳三糖酶蛋白 3 样蛋白 1（CHI3L1）和 CD31 标记的微血管密度（MVD）在宫颈鳞状细胞癌（CSCC）中的表达，并评估其预后影响。
采用 Elivision™ 免疫组化法检测不同宫颈组织中 CHI3L1 的表达和 MVD。分析 CSCC 组织中 CHI3L1 与 MVD 的关系，并探讨 CHI3L1、MVD 与临床病理参数的关系。采用单因素和多因素生存分析评估对无进展生存期（PFS）和总生存期（OS）的影响。
CSCC 组织中 CHI3L1 蛋白的阳性表达率（69.9%，72/103）明显高于高级别宫颈上皮内瘤变（53.3%，32/60）、低级别宫颈上皮内瘤变（25%，15/60）和正常宫颈组织（16.7%，10/60）。CSCC 的 MVD 值范围为 6 至 64，正常宫颈组织、高级别上皮内病变或低级别上皮内病变均未见微血管形成。CHI3L1 和 MVD 的高表达与 CSCC 的浸润深度、分化程度、血管侵犯和淋巴结转移明显相关（均<0.05）。在 CSCC 中，CHI3L1 高表达组（41.35±9.056）的 MVD 表达明显高于 CHI3L1 低表达组（23.26±11.000，<0.05）。单因素 Kaplan-Meier 分析显示，FIGO 分期、肿瘤直径、淋巴结转移、血管侵犯、CSCC 的 CHI3L1 和 MVD 与 PFS 和 OS 的预后相关（均<0.05）；然而，CHI3L1 和 MVD 不是独立的预后因素。
CHI3L1 可能参与宫颈癌的进展。其高表达可促进肿瘤微环境中的新生血管形成。CHI3L1 可能是宫颈癌潜在的治疗靶点。
