College of Biological Sciences, China Agricultural University, Beijing Haidian, China.
Plant Signal Behav. 2022 Dec 31;17(1):2092699. doi: 10.1080/15592324.2022.2092699.
The regulation of hypocotyl elongation is an important process in plant growth and development and depends on the activity of the plasma membrane (PM) H-ATPase. In this study, we found that the Arabidopsis protein SOS3-LIKE CALCIUM BINDING PROTEIN3 (SCaBP3) negatively regulates PM H-ATPase activity in yeast and hypocotyl elongation in dark-grown seedlings. Yeast two-hybrid assays showed that SCaBP3 interacts with representative members of the Arabidopsis PM H-ATPase family. Experiments in RS-72 yeast showed that SCaBP3 negatively regulates PM H-ATPase activity-dependent yeast cell growth. Hypocotyl elongation was promoted in the loss-of-function mutant and inhibited in overexpression lines of Arabidopsis. We propose that SCaBP3 modulates hypocotyl elongation by negatively regulating PM H-ATPase activity.
下胚轴伸长的调控是植物生长发育的一个重要过程，依赖于质膜（PM）H+-ATPase 的活性。在这项研究中，我们发现拟南芥蛋白 SOS3-LIKE CALCIUM BINDING PROTEIN3（SCaBP3）负调控酵母中的 PM H+-ATPase 活性和暗培养幼苗的下胚轴伸长。酵母双杂交实验表明，SCaBP3 与拟南芥 PM H+-ATPase 家族的代表性成员相互作用。在 RS-72 酵母中的实验表明，SCaBP3 负调控 PM H+-ATPase 活性依赖性酵母细胞生长。拟南芥功能丧失突变体 中促进了下胚轴伸长，而过表达系中则抑制了下胚轴伸长。我们提出 SCaBP3 通过负调控 PM H+-ATPase 活性来调节下胚轴伸长。
