Department of Orthodontics, Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, P.R. China.
Mol Med Rep. 2022 Aug;26(2). doi: 10.3892/mmr.2022.12782. Epub 2022 Jun 28.
Mangiferin (MAG) is a polyphenolic compound present in mangoes. This compound suppresses inflammation and decreases bone destruction. This study aimed to determine whether MAG directly promotes proliferation and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs). Cell proliferation and osteogenic differentiation experiments were performed in hPDLSCs, and MAG was used as a stimulator during osteogenic induction. Alkaline phosphatase (ALP) activity and Alizarin red staining were analyzed, and the expression of osteogenesis‑associated genes was investigated by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blot analysis to determine the effect of MAG on the osteogenic differentiation of hPDLSCs. Galunisertib was used to selectively inhibit TGF‑β/SMAD2 signaling. Western blotting was performed to study the underlying mechanism. Cell Counting Kit‑8 assay showed that MAG did not promote the proliferation of hPDLSCs. MAG (200 µM) significantly promoted ALP activity, mRNA levels of alkaline phosphatase biomineralization associated, collagen type 1, and runt‑related transcription factor‑2, protein levels of SMAD5, alkaline phosphatase and bone morphogenetic protein 2 protein expression and mineralized nodule formation in hPDLSCs. Furthermore, MAG significantly promoted the phosphorylation of SMAD2. Galunisertib inhibited the activation of SMAD2 and partially reversed the MAG‑mediated promotion of hPDLSC osteogenic differentiation. These data indicated that MAG promoted osteogenic differentiation of hPDLSCs potentially through TGF‑β/SMAD2 signaling. Therefore, MAG may help improve periodontal bone loss.
芒果苷（MAG）是存在于芒果中的一种多酚化合物。这种化合物可以抑制炎症并减少骨质破坏。本研究旨在确定 MAG 是否直接促进人牙周膜干细胞（hPDLSCs）的增殖和成骨分化。在 hPDLSCs 中进行了细胞增殖和成骨分化实验，并在成骨诱导过程中使用 MAG 作为刺激物。分析碱性磷酸酶（ALP）活性和茜素红染色，并通过逆转录-定量聚合酶链反应（RT-qPCR）和 Western blot 分析研究成骨相关基因的表达，以确定 MAG 对 hPDLSCs 成骨分化的影响。Galunisertib 用于选择性抑制 TGF-β/SMAD2 信号。进行 Western blot 分析以研究潜在机制。细胞计数试剂盒-8 测定法显示 MAG 不会促进 hPDLSCs 的增殖。MAG（200μM）可显著促进 ALP 活性、碱性磷酸酶生物矿化相关、胶原 I 和 runt 相关转录因子-2 的 mRNA 水平、SMAD5、碱性磷酸酶和骨形态发生蛋白 2 蛋白表达水平和矿化结节形成。此外，MAG 可显著促进 SMAD2 的磷酸化。Galunisertib 抑制 SMAD2 的激活，并部分逆转 MAG 介导的 hPDLSC 成骨分化促进作用。这些数据表明 MAG 通过 TGF-β/SMAD2 信号促进 hPDLSCs 的成骨分化。因此，MAG 可能有助于改善牙周骨丢失。
