College of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables, Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou, P. R. China.
J Food Biochem. 2022 Oct;46(10):e14285. doi: 10.1111/jfbc.14285. Epub 2022 Jun 28.
Changes in quality attributes, ascorbate-glutathione (AsA-GSH) cycle, and melatonin (MLT) synthesis were evaluated in cherry tomato fruit treated with MLT solution at 0.1 mM during storage at room temperature for 16 days. According to the results, the MLT treatment was beneficial to maintaining fruit quality as indicated by the declines in weight loss, fruit decay, and titratable acid (TA), accompanied by the maintenance of fruit firmness, total soluble solids (TSS) as well as TSS/TA ratio. Also, the MLT treatment not only effectively inhibited oxidative damage via reducing relative electrolyte leakage and malondialdehyde content, but also improved antioxidant capacity via stimulating AsA-GSH cycle. Moreover, the MLT treatment promoted endogenous MLT synthesis by upregulating the expressions of biosynthetic genes consisting of SlTDC, SlT5H, SlSNAT, and SlASMLT. Thus, our results suggested that the MLT treatment might be involved in maintaining quality in cherry tomato fruit during room temperature by promoting antioxidant capacity and enhancing endogenous MLT. PRACTICAL APPLICATIONS: As a typical climacteric fruit, cherry tomato fruit ripen rapidly and are easily infected by various pathogenic fungi during storage under ambient conditions, which leads to short storage life and a decrease in economic value. The results showed that the application of MLT maintained cherry tomato quality via improving antioxidant capacities and enhancing endogenous MLT. Therefore, MLT treatment could become a promising postharvest strategy for quality maintenance in cherry tomatoes during room storage.
在室温下储存 16 天后，用 0.1mM 的 MLT 溶液处理樱桃番茄果实，评估其品质属性、抗坏血酸-谷胱甘肽（AsA-GSH）循环和褪黑素（MLT）合成的变化。结果表明，MLT 处理有利于维持果实品质，表现为失重、果实腐烂和可滴定酸（TA）下降，同时保持果实硬度、总可溶性固体（TSS）和 TSS/TA 比。此外，MLT 处理不仅通过降低相对电导率泄漏和丙二醛含量有效抑制氧化损伤，还通过刺激 AsA-GSH 循环来提高抗氧化能力。此外，MLT 处理通过上调由 SlTDC、SlT5H、SlSNAT 和 SlASMLT 组成的生物合成基因的表达来促进内源性 MLT 合成。因此，我们的结果表明，MLT 处理可能通过增强抗氧化能力和增强内源性 MLT 参与维持樱桃番茄果实在室温下的品质。 实用程序：作为一种典型的呼吸跃变型果实，樱桃番茄果实在常温下贮藏时迅速成熟，容易感染各种病原菌，导致贮藏寿命短，经济价值下降。结果表明，MLT 的应用通过提高抗氧化能力和增强内源性 MLT 来维持樱桃番茄的品质。因此，MLT 处理可能成为室温下樱桃番茄贮藏期维持品质的一种有前途的采后策略。
