School of Food and Biological Engineering, Key Laboratory for Agricultural Products, Processing of Anhui Province, Hefei University of Technology, Hefei 230601, China.
School of Food and Biological Engineering, Key Laboratory for Agricultural Products, Processing of Anhui Province, Hefei University of Technology, Hefei 230601, China.
Food Chem. 2022 Nov 30;395:133562. doi: 10.1016/j.foodchem.2022.133562. Epub 2022 Jun 23.
In this study, glycated soy β-conglycinin (β-CG) stabilized curcumin (Cur) composites were fabricated by a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX). Intrinsic fluorescence and far-UV CD spectra revealed that glycation did not affect the self-assembly property of β-CG in the pH-shifting treatment. The structure of β-CG-DEX could be unfolded at pH 12.0 and reassembled during acidification (from pH 12.0 to 7.0). Meanwhile, β-CG-DEX-3d, which was incubated at 60 °C for 3 days, exhibited a high loading capacity (123.4 mg/g) for curcumin, which far exceeds that (74.90 mg/g) of β-CG-Cur. Moreover, the reassembled β-CG-DEX-3d-Cur showed eminent antioxidant activity of approximately 1.5 times higher than that of free curcumin. During the simulated gastrointestinal condition, compared with β-CG-Cur, β-CG-DEX-3d-Cur nanoparticles showed a more stable and sustained release of curcumin. Thus, β-CG-DEX has immense potential to become a new delivery carrier for hydrophobic food components by means of a self-assembly strategy.
在这项研究中，通过β-伴大豆球蛋白-葡聚糖缀合物（β-CG-DEX）的独特可逆自组装特性，制备了糖化大豆β-伴大豆球蛋白（β-CG）稳定的姜黄素（Cur）复合材料。内源性荧光和远紫外 CD 光谱表明，糖化在 pH 转换处理中不影响 β-CG 的自组装特性。在 pH 12.0 下可以展开β-CG-DEX 的结构，并在酸化过程中重新组装（从 pH 12.0 到 7.0）。同时，在 60°C 下孵育 3 天的β-CG-DEX-3d 对姜黄素的载药量（123.4mg/g）很高，远远超过β-CG-Cur 的载药量（74.90mg/g）。此外，重新组装的β-CG-DEX-3d-Cur 表现出的抗氧化活性约为游离姜黄素的 1.5 倍。在模拟胃肠道条件下，与β-CG-Cur 相比，β-CG-DEX-3d-Cur 纳米粒子表现出更稳定和持续释放姜黄素的特性。因此，β-CG-DEX 具有通过自组装策略成为疏水食物成分的新型递送载体的巨大潜力。
