Alam I, Silver M J
Thromb Res. 1987 Feb 15;45(4):311-22. doi: 10.1016/0049-3848(87)90220-9.
Washed human platelets were incubated with radioactive 1-[3H]alkyl-2-hydroxyglycero-3-phosphocholine (lyso-PAF) at 37 degrees C. [3H]lyso-PAF was converted by platelets into [3H]alkylacyl-GPC which was incorporated. Incorporation of radioactivity was time dependent and reached a maximum of 57 percent in one h. This formation and incorporation of [3H]alkylacyl-GPC was inhibited (50%) by extracellular calcium (1.3 mM). Labeled platelets were treated for 5 min with either thrombin (2.5 U/ml) or saline solution. While there was no change in the saline control, thrombin induced a reduction in the content of [3H]alkylacyl-GPC, accompanied by an increase in [3H]lyso-PAF presumably by stimulation of phospholipase A2. There was no apparent increase in radioactivity comigrating with PAF. This was probably due to the overwhelming dilution of the radioactive alkylacyl-GPC by the endogenous nonradioactive compound (ratio-1/3200). These studies suggest that human platelets can take up lyso-PAF and acylate it to alkylacyl-GPC which is susceptible to phospholipase A2 activity.
洗涤过的人血小板在37℃下与放射性1-[³H]烷基-2-羟基甘油-3-磷酸胆碱（溶血血小板活化因子）一起孵育。血小板将[³H]溶血血小板活化因子转化为[³H]烷基酰基甘油磷酸胆碱并将其掺入。放射性的掺入呈时间依赖性，1小时内达到最大值57%。细胞外钙（1.3 mM）抑制（50%）[³H]烷基酰基甘油磷酸胆碱的这种形成和掺入。用凝血酶（2.5 U/ml）或盐溶液处理标记的血小板5分钟。盐溶液对照组无变化，而凝血酶诱导[³H]烷基酰基甘油磷酸胆碱含量降低，同时[³H]溶血血小板活化因子可能因磷脂酶A2的刺激而增加。与血小板活化因子共迁移的放射性没有明显增加。这可能是由于内源性非放射性化合物（比例为1/3200）对放射性烷基酰基甘油磷酸胆碱的过度稀释。这些研究表明，人血小板可以摄取溶血血小板活化因子并将其酰化为对磷脂酶A2活性敏感的烷基酰基甘油磷酸胆碱。
