Universidad Privada Antenor Orrego, La Libertad, Trujillo, Perú.
Laboratorio de Referencia Regional La Libertad, Perú.
Rev Peru Med Exp Salud Publica. 2022 Jan-Mar;39(1):77-82. doi: 10.17843/rpmesp.2022.391.10136. Epub 2022 Jun 24.
Sparteine is an alkaloid with bacteriostatic activity on the genus Mycobacterium. The aim of this study was to evaluate the antimicrobial activity of sparteine on the growth of 4 ATCC strains of Mycobacterium tuberculosis (susceptible, resistant to isoniazid, resistant to rifampicin and multidrug-resistant) in vitro. Validation of bactericidal activity of sparteine sulfate was carried out through an adaptation of the Microscopic-Observation Drug-Susceptibility (MODS) method according to the guidelines of the Peruvian National Health Institute. The results demonstrate that at concentrations of 25; 50 and 100 Mm of sparteine sulfate, there is no development of colony-forming units in any of the 4 evaluated strains. Our results demonstrate the potential in vitro antimicrobial effect of sparteine on multidrug-resistant tuberculosis.
斯帕丁是一种对分枝杆菌属具有抑菌活性的生物碱。本研究旨在评估硫酸斯帕丁对体外培养的 4 株结核分枝杆菌（敏感、异烟肼耐药、利福平耐药和耐多药）生长的抗菌活性。硫酸斯帕丁杀菌活性的验证是通过根据秘鲁国家卫生研究所的指南改编微观药物敏感性（MODS）方法进行的。结果表明，在 25 ；50 和 100μm 的硫酸斯帕丁浓度下，在评估的 4 株菌株中，没有形成菌落形成单位。我们的结果表明硫酸斯帕丁对耐多药肺结核具有潜在的体外抗菌作用。
