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一种基于嵌合型热稳定M2e和H3茎部的甲型流感病毒通用疫苗。

A chimeric thermostable M2e and H3 stalk-based universal influenza A virus vaccine.

作者信息

Subbiah Jeeva, Oh Judy, Kim Ki-Hye, Shin Chong-Hyun, Park Bo Ryoung, Bhatnagar Noopur, Seong Baik-Lin, Wang Bao-Zhong, Kang Sang-Moo

机构信息

Center for Inflammation, Immunity & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA, 30303, USA.

Department of Microbiology, College of Medicine, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.

出版信息

NPJ Vaccines. 2022 Jun 29;7(1):68. doi: 10.1038/s41541-022-00498-6.

DOI:10.1038/s41541-022-00498-6
PMID:35768475
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9243060/
Abstract

We developed a new chimeric M2e and H3 hemagglutinin (HA) stalk protein vaccine (M2e-H3 stalk) by genetic engineering of modified H3 stalk domain conjugated with conserved M2e epitopes to overcome the drawbacks of low efficacy by monomeric domain-based universal vaccines. M2e-H3 stalk protein expressed and purified from Escherichia coli was thermostable, displaying native-like antigenic epitopes recognized by antisera of different HA subtype proteins and influenza A virus infections. Adjuvanted M2e-H3 stalk vaccination induced M2e and stalk-specific IgG antibodies recognizing viral antigens on virus particles and on the infected cell surface, CD4 and CD8 T-cell responses, and antibody-dependent cytotoxic cell surrogate activity in mice. M2e-H3 stalk was found to confer protection against heterologous and heterosubtypic cross-group subtype viruses (H1N1, H5N1, H9N2, H3N2, H7N9) at similar levels in adult and aged mice. These results provide evidence that M2e-H3 stalk chimeric proteins can be developed as a universal influenza A virus vaccine candidate for young and aged populations.

摘要

我们通过对修饰的H3茎域进行基因工程改造，并将其与保守的M2e表位偶联，开发了一种新型嵌合M2e和H3血凝素（HA）茎蛋白疫苗（M2e-H3茎），以克服基于单体结构域的通用疫苗效力低下的缺点。从大肠杆菌中表达并纯化的M2e-H3茎蛋白具有热稳定性，展现出可被不同HA亚型蛋白抗血清和甲型流感病毒感染识别的类似天然的抗原表位。佐剂化的M2e-H3茎疫苗接种可诱导产生识别病毒颗粒和感染细胞表面病毒抗原的M2e和茎特异性IgG抗体、CD4和CD8 T细胞应答，以及小鼠体内抗体依赖性细胞毒性细胞替代活性。结果发现，M2e-H3茎在成年和老年小鼠中对异源和异亚型跨组亚型病毒（H1N1、H5N1、H9N2、H3N2、H7N9）具有相似水平的保护作用。这些结果证明，M2e-H3茎嵌合蛋白可开发成为针对年轻和老年人群的通用甲型流感病毒候选疫苗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/8618c3783cd9/41541_2022_498_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/9b34a8b976aa/41541_2022_498_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/8f0383406e2e/41541_2022_498_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/225b51634994/41541_2022_498_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/d0de52512d2f/41541_2022_498_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/99df540af3e5/41541_2022_498_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/7ce8f35cba2e/41541_2022_498_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/623fa5e806d6/41541_2022_498_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/a69c61875240/41541_2022_498_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/8618c3783cd9/41541_2022_498_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/9b34a8b976aa/41541_2022_498_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/8f0383406e2e/41541_2022_498_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/225b51634994/41541_2022_498_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/d0de52512d2f/41541_2022_498_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/99df540af3e5/41541_2022_498_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/7ce8f35cba2e/41541_2022_498_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/623fa5e806d6/41541_2022_498_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/a69c61875240/41541_2022_498_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e36/9243060/8618c3783cd9/41541_2022_498_Fig9_HTML.jpg

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本文引用的文献

1
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Science. 2020 Jun 19;368(6497):1335-1340. doi: 10.1126/science.aaz5143.
2
Universal monoclonal antibody-based influenza hemagglutinin quantitative enzyme-linked immunosorbent assay.通用单克隆抗体流感血凝素定量酶联免疫吸附测定法。
Vaccine. 2019 Mar 7;37(11):1457-1466. doi: 10.1016/j.vaccine.2019.01.068. Epub 2019 Feb 11.
3
Universal Influenza Virus Vaccines That Target the Conserved Hemagglutinin Stalk and Conserved Sites in the Head Domain.
乙型流感病毒神经氨酸酶mRNA疫苗通过补充灭活裂解疫苗增强跨谱系保护的双重作用。
J Virol. 2025 May 20;99(5):e0229424. doi: 10.1128/jvi.02294-24. Epub 2025 Apr 23.
4
Design, Synthesis and Evaluation of a Candidate Fusion Epitopic Construct Vaccine Based on M2e, HA1, HA2, NA and NP Fragments of the Highly Pathogenic Avian H5N1 Influenza Virus.基于高致病性H5N1禽流感病毒M2e、HA1、HA2、NA和NP片段的候选融合表位构建疫苗的设计、合成与评价
Arch Razi Inst. 2024 Aug 1;79(4):849-856. doi: 10.32592/ARI.2024.79.4.849. eCollection 2024 Aug.
5
Addressing unexpected bacterial RNA safety concerns of E. coli produced influenza NP through CpG loaded mutant.通过负载CpG的突变体解决大肠杆菌产生的流感核蛋白意外的细菌RNA安全性问题。
NPJ Vaccines. 2025 Feb 15;10(1):32. doi: 10.1038/s41541-025-01087-z.
6
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Vaccines (Basel). 2024 Dec 30;13(1):22. doi: 10.3390/vaccines13010022.
7
A multi-antigen vaccinia vaccine broadly protected mice against SARS-CoV-2 and influenza A virus while also targeting SARS-CoV-1 and MERS-CoV.一种多抗原痘苗病毒疫苗可广泛保护小鼠免受严重急性呼吸综合征冠状病毒2（SARS-CoV-2）和甲型流感病毒感染，同时还能靶向严重急性呼吸综合征冠状病毒1（SARS-CoV-1）和中东呼吸综合征冠状病毒（MERS-CoV）。
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Poult Sci. 2025 Jan;104(1):104616. doi: 10.1016/j.psj.2024.104616. Epub 2024 Dec 1.
9
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Vaccines (Basel). 2024 Oct 18;12(10):1187. doi: 10.3390/vaccines12101187.
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Front Immunol. 2024 Oct 8;15:1483720. doi: 10.3389/fimmu.2024.1483720. eCollection 2024.
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J Infect Dis. 2019 Apr 8;219(Suppl_1):S62-S67. doi: 10.1093/infdis/jiy711.
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5
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6
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7
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8
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