Kennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, United Kingdom.
Medical Inflammation Research, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
Front Immunol. 2022 Jun 13;13:892251. doi: 10.3389/fimmu.2022.892251. eCollection 2022.
Autoimmune murine disease models are vital tools for identifying novel targets and finding better treatments for human diseases. Complete Freund's adjuvant is commonly used to induce disease in autoimmune models, and the quality of the adjuvant/autoantigen emulsion is of critical importance in determining reproducibility. We have established an emulsification method using a standard homogenizer and specially designed receptacle. Emulsions are easy to prepare, form stable and uniform water-in-oil particles, are faster to make than the traditional syringe method, use less material and are designed to fill syringes with ease. In the present study, we have validated the emulsions for induction of experimental autoimmune encephalitis, collagen II induced arthritis, antigen induced arthritis, and delayed type hypersensitivity models. These models were induced consistently and reproducibly and, in some cases, the new method outperformed the traditional method. The method described herein is simple, cost-effective and will reduce variability, thereby requiring fewer animals for research involving animal models of autoimmune disease and in vaccine development.
自身免疫性疾病小鼠模型是鉴定新靶点和寻找人类疾病更好治疗方法的重要工具。完全弗氏佐剂常用于诱导自身免疫模型疾病，佐剂/自身抗原乳剂的质量对确定可重复性至关重要。我们使用标准匀浆器和专门设计的容器建立了一种乳化方法。乳化液易于制备，形成稳定且均匀的油包水颗粒，比传统的注射器方法更快，使用的材料更少，并且设计用于轻松填充注射器。在本研究中，我们验证了用于诱导实验性自身免疫性脑脊髓炎、胶原 II 诱导性关节炎、抗原诱导性关节炎和迟发型超敏反应模型的乳化液。这些模型的诱导一致且可重复，在某些情况下，新方法优于传统方法。本文所述的方法简单、经济有效，可减少变异性，从而在涉及自身免疫性疾病动物模型和疫苗开发的研究中减少所需动物的数量。
