Chinese Academy of Sciences, National Center for Magnetic Resonance in Wuhan, Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Wuhan, 430071, People's Republic of China.
University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
Biomol NMR Assign. 2022 Oct;16(2):325-332. doi: 10.1007/s12104-022-10098-z. Epub 2022 Jun 30.
The Sugar Will Eventually be Exported Transporter (SWEET) family is a new class of transporters that plays crucial roles in the cellular sugar transport process. Their bacterial homologs, known as SemiSWEETs, are among the smallest transporters and can be used as a prototype for studying the biological properties of sugar transporters. Here, a set of dipolar-based multidimensional solid-state NMR spectra were employed to investigate the structure of Vibrio sp. SemiSWEET (Vs-SemiSWEET) reconstituted in the native-like lipid bilayers. A nearly complete (88% of the amino acid residues) backbone and side-chain C and N chemical shift assignments of Vs-SemiSWEET were obtained. The overall secondary structure of Vs-SemiSWEET predicted from the obtained C and N chemical shifts is similar to that from X-ray crystallography, with some differences, reflecting the influence of the membrane environments to the structure of membrane proteins.
糖最终会被输出转运蛋白（SWEET）家族是一类新的转运蛋白，在细胞的糖转运过程中起着关键作用。它们的细菌同源物被称为 SemiSWEET，是最小的转运蛋白之一，可以作为研究糖转运蛋白生物特性的原型。在这里，使用了一组基于偶极子的多维固态 NMR 谱来研究在类似天然的脂质双层中重建的 Vibrio sp. SemiSWEET（Vs-SemiSWEET）的结构。获得了 Vs-SemiSWEET 的几乎完整的（88%的氨基酸残基）骨架和侧链 C 和 N 化学位移分配。从获得的 C 和 N 化学位移预测的 Vs-SemiSWEET 的整体二级结构与 X 射线晶体学的结果相似，存在一些差异，反映了膜环境对膜蛋白结构的影响。
