Hiding in Plain Sight: Rain Water Puddles in Nicobar Islands of India Reveal Abundance of a New Frog Species of the Genus Tschudi, 1838 (Anura: Microhylidae).

Recent studies on frogs of the species complex have demonstrated that high genetic variation exists among its various known populations from regions across Asia. We assessed the taxonomic identity of the Nicobar population of cf. and compared it to the typical from Taiwan and the two recently described species in this complex from Vietnam. Our study demonstrates that the Nicobar population is both genetically and morphologically divergent and warrants recognition as a new species, which we formally describe here as sp. nov. The new species is closely related to , , and , but diagnosable from all three species by a suite of morphological characters, such as the presence of two small tubercles at mid-dorsum along with ( )-shaped markings, length of finger I longer than half the length of finger II, presence of three distinct metacarpal tubercles on hand, rudimentary foot webbing, as well as its slender body shape, granular dorsal skin texture, and other colour characters and body markings. Statistical analyses based on multiple morphometric characters also clearly separate our new taxon from , with which it was previously confused. Our phylogenetic analyses based on the mitochondrial 16S rRNA locus find sp. nov. to be nested in the species group, where it is delimited as a distinct species. This lineage shows genetic distances of ≥ 3.5% from all the other known congeners. Currently, the known distribution of the new taxon is restricted to the southernmost group of Nicobar Islands-Great Nicobar, Kondul, and Little Nicobar-where it is found in abundance across a wide range of habitats during the monsoon season. Our study underlines the need to reassess the identity of all the known populations of '' from other regions in Southeast and East Asia. To facilitate future taxonomic work in the light of our and other recent findings, we also provide a detailed redescription and revised diagnosis for based on morphological examination of its century-old type material originating from Taiwan.