Bai Yupeng, Zhang Mingjing, Peng Sheng, Wang Yuting, Gu Ye, Fang Qianqian, Hu Liqun
Department of Cardiology, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Inner Mongolia Conba Pharmaceutical Co., Ltd., Hangzhou, China.
Front Physiol. 2022 Jun 14;13:913399. doi: 10.3389/fphys.2022.913399. eCollection 2022.
Preliminary clinical studies have confirmed that Shexiang Tongxin dropping pills (STDPs) could improve angina pectoris and attenuate vascular endothelial dysfunction in patients with slow coronary flow, but the underlying mechanism is not fully unclear. We aimed to investigate the impact of STDP in a swine model of coronary slow flow (SF) and related mechanisms. SF was induced by coronary injection of microspheres, and pigs were randomly divided into the SF group and SF plus STDP group. Pigs in the STDP group received sublingual STDP for 10 min, followed by 1 g STDP oral administration daily for 6 days. Coronary angiography was performed, the TIMI frame count (TFC) was determined, and hemodynamic measurements were performed before, at 30 min, and 7 days post-SF. Serum levels of total NO, NOS, ET-1, C-TNI, and BNP were measured. Myocardial expressions of TNF and IL-6, eNOS, VEGF, CD31, and α-SMA were analyzed by immunohistochemistry and Western blotting. Compared to the SF group, LVEF and TFC were significantly improved at 7 days post-SF in the STDP group. The serum ET-1 level was significantly reduced at 7 days, and NO and NOS levels were significantly higher in the STDP group. Seven days post-SF, myocardial TNF and IL-6 expressions were significantly downregulated, while the expressions of eNOS and VEGF, CD31, and ɑ-SMA were significantly upregulated in the STDP group. Our results showed that STDP improved cardiac function and coronary flow, possibly through reducing inflammatory responses and upregulating myocardial eNOS and VEGF, CD31, and the ɑ-SMA expression
初步临床研究证实，麝香通心滴丸（STDPs）可改善冠状动脉血流缓慢患者的心绞痛并减轻血管内皮功能障碍，但其潜在机制尚不完全清楚。我们旨在研究STDP对猪冠状动脉慢血流（SF）模型的影响及相关机制。通过冠状动脉注射微球诱导SF，将猪随机分为SF组和SF加STDP组。STDP组猪舌下含服STDP 10分钟，随后每天口服1克STDP，共6天。进行冠状动脉造影，测定TIMI帧计数（TFC），并在SF前、30分钟和7天后进行血流动力学测量。检测血清总NO、NOS、ET-1、C-TNI和BNP水平。通过免疫组织化学和蛋白质印迹法分析心肌中TNF和IL-6、eNOS、VEGF、CD31和α-SMA的表达。与SF组相比，STDP组在SF后7天LVEF和TFC显著改善。7天时血清ET-1水平显著降低，STDP组NO和NOS水平显著升高。SF后7天，STDP组心肌TNF和IL-6表达显著下调，而eNOS、VEGF、CD31和ɑ-SMA表达显著上调。我们的结果表明，STDP可能通过减少炎症反应和上调心肌eNOS、VEGF、CD31和ɑ-SMA表达来改善心脏功能和冠状动脉血流。
