Kobayashi Pharmaceutical Co., Ltd., 1-30-3, Toyokawa, Ibaraki-city, Osaka, 567-0057, Japan.
Tokyo University of Technology, Graduate School of Bionics, Computer and Media Sciences, 1404-1, Katakuramachi, Hachioji-city, Tokyo, 192-0982, Japan.
Biosci Biotechnol Biochem. 2022 Aug 24;86(9):1240-1246. doi: 10.1093/bbb/zbac107.
Intercellular lipids comprise mainly ceramides, known to enhance the barrier function of the stratum corneum. However, the activities of ceramides inside the skin have not yet been fully elucidated. Here we examined how the human ceramide mixture (HC123) functions in the dermis. We treated human skin fibroblasts with HC123-expressed fibroblast growth factor (FGF), transforming growth factor-β (TGF-β), collagen I, and fibrillin. We found that HC123 promoted the formation of collagen fibers and microfibrils (fibrillin) which affect the elasticity of the skin. We also confirmed that the gene expression of collagen and fibrillin is promoted via TGF-β and FGF2, respectively. We then investigated the permeability of HC123 for external use, in pursuit of evidence that HC123 may exert an anti-aging effect by penetrating into the dermis, activating fibroblasts, and promoting the production of collagen fibers and elastin-related microfibrils.
细胞间脂质主要由神经酰胺组成，已知神经酰胺可增强角质层的屏障功能。然而，皮肤内神经酰胺的活性尚未被充分阐明。在这里，我们研究了人神经酰胺混合物（HC123）在真皮中的作用。我们用 HC123 表达的成纤维细胞生长因子（FGF）、转化生长因子-β（TGF-β）、I 型胶原和原纤维蛋白处理人皮肤成纤维细胞。我们发现 HC123 促进了影响皮肤弹性的胶原纤维和微纤维（原纤维蛋白）的形成。我们还证实，胶原和原纤维蛋白的基因表达分别通过 TGF-β和 FGF2 促进。然后，我们研究了 HC123 外用的通透性，以期证明 HC123 可能通过渗透到真皮、激活成纤维细胞、促进胶原纤维和与弹性蛋白相关的微纤维的产生来发挥抗衰老作用。
