Center of Reproductive Medicine, Affiliated Children's Hospital of Shanxi & Women Health Center of Shanxi Medicine University, Taiyuan, Shanxi, China.
Medicine (Baltimore). 2022 Jul 1;101(26):e29774. doi: 10.1097/MD.0000000000029774.
Embryo chromosomal abnormalities are considered as the main cause of low pregnancy rate for in vitro fertilization (IVF). Recently, a new metric of success in assisted reproductive technology, that is, the ability to achieve at least 1 euploid blastocyst for transfer, has been brought into focus among clinicians. Our study aimed to investigate the effects of different factors on the euploidy of blastocysts undergoing IVF and preimplantation genetic testing (PGT). This retrospective observational study included 493 cycles underwent IVF/intracytroplasmatic sperm injection intended to obtain trophectoderm biopsy for PGT from June 2016 to December 2019 at a single academic fertility center. Logistic regression was adopted to analyze the clinical characteristics and embryonic data related to the ability to achieve at least 1 euploid blastocyst for transfer. The study took 1471 blastocysts from 493 cycles as samples for PGT. Among them, 149 cycles (30.22%) had no euploid blastocyst and 344 cycles (69.78%) had at least 1 euploid blastocyst. A multivariate logistic analysis suggested that maternal age >36, abnormal parental karyotype, nonfirst cycles and blastocysts number per cycle <3 were the risk factors for no euploid blastocyst. The parental karyotype, maternal age, number of cycles, and number of blastocysts per cycle were the dominant factors affecting the ability to achieve at least 1 euploid blastocyst for transfer and therefore could be regarded as potential predictors for genetic counseling.
胚胎染色体异常被认为是体外受精（IVF）低妊娠率的主要原因。最近，辅助生殖技术的一种新的成功指标，即有能力获得至少 1 个用于移植的整倍体囊胚，已成为临床医生关注的焦点。我们的研究旨在探讨不同因素对 IVF 和植入前遗传学检测（PGT）中囊胚整倍体的影响。这项回顾性观察研究纳入了 2016 年 6 月至 2019 年 12 月在一家学术生育中心进行的 493 个接受 IVF/胞浆内精子注射以获得滋养外胚层活检进行 PGT 的周期。采用逻辑回归分析与获得至少 1 个用于移植的整倍体囊胚能力相关的临床特征和胚胎数据。该研究对 493 个周期的 1471 个囊胚进行了 PGT。其中，149 个周期（30.22%）没有整倍体囊胚，344 个周期（69.78%）有至少 1 个整倍体囊胚。多变量逻辑分析表明，母亲年龄>36 岁、异常的父母染色体核型、非首次周期和每个周期的囊胚数<3 是没有整倍体囊胚的危险因素。父母染色体核型、母亲年龄、周期数和每个周期的囊胚数是影响获得至少 1 个整倍体囊胚能力的主要因素，因此可以作为遗传咨询的潜在预测因素。
