Center for Lasers and Photonics, IIT Kanpur, Kanpur, 208016, India.
Department of Biological sciences and Bioengineering, IIT Kanpur, Kanpur, 208016, India.
Sci Rep. 2022 Jul 1;12(1):11192. doi: 10.1038/s41598-022-15007-x.
Fluorescence spectroscopy has the potential to identify discriminatory signatures, crucial for early diagnosis of cervical cancer. We demonstrate here the design, fabrication and testing of a 3D printed smartphone based spectroscopic device. Polarized fluorescence and elastic scattering spectra are captured through the device using a 405 nm laser and a white LED source respectively. The device has been calibrated by comparison of spectra of standard fluorophores (Flavin adenine dinucleotide, fluorescein, rhodamine, and porphyrin) with the corresponding spectra collected from a commercial spectrometer. A few cervical tissue spectra have also been captured for proof of its applicability as a portable, standalone device for the collection of intrinsic fluorescence spectra from human cervix.
荧光光谱学有可能识别出具有鉴别意义的特征，这对于早期诊断宫颈癌至关重要。我们在此展示了一种基于智能手机的 3D 打印光谱仪的设计、制造和测试。该设备使用 405nm 激光和白色 LED 源分别捕获偏振荧光和弹性散射光谱。通过将标准荧光团（黄素腺嘌呤二核苷酸、荧光素、罗丹明和卟啉）的光谱与从商业光谱仪收集到的相应光谱进行比较，对该设备进行了校准。还捕获了一些宫颈组织光谱，以证明其作为一种便携式、独立式设备，可从人宫颈采集固有荧光光谱的适用性。
