General and Molecular Microbiology, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University of Oldenburg, Oldenburg, Germany.
In-Vitro-Diagnostics, Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Stuttgart, Germany.
Proteomics. 2022 Sep;22(18):e2100404. doi: 10.1002/pmic.202100404. Epub 2022 Jul 26.
The Antarctic krill (Euphausia superba Dana) is a keystone species in the Southern Ocean that uses an arsenal of hydrolases for biomacromolecule decomposition to effectively digest its omnivorous diet. The present study builds on a hybrid-assembled transcriptome (13,671 ORFs) combined with comprehensive proteome profiling. The analysis of individual krill compartments allowed detection of significantly more different proteins compared to that of the entire animal (1464 vs. 294 proteins). The nearby krill sampling stations in the Bransfield Strait (Antarctic Peninsula) yielded rather uniform proteome datasets. Proteins related to energy production and lipid degradation were particularly abundant in the abdomen, agreeing with the high energy demand of muscle tissue. A total of 378 different biomacromolecule hydrolysing enzymes were detected, including 250 proteases, 99 CAZymes, 14 nucleases and 15 lipases. The large repertoire in proteases is in accord with the protein-rich diet affiliated with E. superba's omnivorous lifestyle and complex biology. The richness in chitin-degrading enzymes allows not only digestion of zooplankton diet, but also the utilisation of the discharged exoskeleton after moulting.
南极磷虾（Euphausia superba Dana）是南大洋的关键物种，它利用一系列的水解酶来分解生物大分子，以有效地消化其杂食性的饮食。本研究基于一个混合组装的转录组（13671 个 ORFs），结合了全面的蛋白质组谱分析。与整个动物相比，对单个磷虾隔室的分析检测到了更多不同的蛋白质（1464 种 vs. 294 种）。在南极半岛的布兰斯菲尔德海峡（Bransfield Strait）附近的磷虾采样站获得了相当均匀的蛋白质组数据集。与能量产生和脂质降解相关的蛋白质在腹部特别丰富，这与肌肉组织的高能量需求相吻合。共检测到 378 种不同的生物大分子水解酶，包括 250 种蛋白酶、99 种 CAZymes、14 种核酸酶和 15 种脂肪酶。蛋白酶的丰富程度与 E. superba 的杂食性生活方式和复杂生物学相关的高蛋白饮食相一致。几丁质降解酶的丰富程度不仅允许消化浮游动物的饮食，还允许在蜕皮后利用排出的外骨骼。
