Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
J Virol Methods. 2022 Sep;307:114573. doi: 10.1016/j.jviromet.2022.114573. Epub 2022 Jun 30.
Pseudorabies virus (PrV) is one of the most important herpesviruses which can cause severe diseases in many mammals and some avian species. In recent years, repeated outbreaks of pseudorabies worldwide indicated an urgent need for new control measures. The results described in this study demonstrated that an extract prepared from the rhizome of Kaempferia galanga L (Kge), which consisted of flavonoids (2.82%), saccharides (61.37%), phenols (1.22%) and saponins (3.10%), possessed a potent anti-PrV activity. In PK-15 cells, Kge treatment inhibited PrV-induced cell death by more than 90% at a dose of 200 μg/mL. The 50% inhibitory concentration (IC) was 55.85 μg/mL. In the PrV-infected mice treated with Kge, the survival rate was up to 60% at day 6 post-infection, while the infected mice without Kge treatment all died. The virus titers in the brains of the Kge-treated infected mice were significantly reduced. Kge treatment also alleviated the severity of the PrV-induced lesions in the heart, liver, spleen, lung and kidney. Kge exhibited immune-regulating activity through the regulation of cytokines (IFN-α, IFN-β, IL-4, IL-6 and TNF-α) in the serum of PrV-infected mice, suggesting that one possible mechanism of anti-PrV activity was through the regulation of immune function. These results suggested that Kge could be a promising drug candidate for treating PrV infections.
伪狂犬病病毒（PrV）是最重要的疱疹病毒之一，可引起许多哺乳动物和某些禽类的严重疾病。近年来，伪狂犬病在全球范围内的反复爆发表明急需采取新的控制措施。本研究的结果表明，从高良姜根茎中制备的提取物（Kge）具有很强的抗 PrV 活性。Kge 由黄酮类化合物（2.82%）、糖类（61.37%）、酚类（1.22%）和皂苷（3.10%）组成，在 200μg/mL 剂量下可抑制超过 90%的 PrV 诱导的细胞死亡。其 50%抑制浓度（IC）为 55.85μg/mL。在 Kge 治疗的 PrV 感染小鼠中，感染后第 6 天的存活率高达 60%，而未用 Kge 治疗的感染小鼠全部死亡。用 Kge 处理可显著降低感染小鼠脑中的病毒滴度。Kge 治疗还减轻了 PrV 感染引起的心脏、肝脏、脾脏、肺和肾脏病变的严重程度。Kge 通过调节感染 PrV 小鼠血清中的细胞因子（IFN-α、IFN-β、IL-4、IL-6 和 TNF-α）表现出免疫调节活性，表明其抗 PrV 活性的一种可能机制是通过调节免疫功能。这些结果表明，Kge 可能是治疗 PrV 感染的有前途的候选药物。
