Mohammadzadeh Farzaneh, Ajdary Marziyeh, Mohammadzadeh Afsaneh, Safdarian Lili, Amidi Fardin, Sobhani Aligholi
Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Endometriosis Research Center, Iran University of Medical Sciences, Tehran, Iran.
Cell Tissue Bank. 2023 Mar;24(1):109-123. doi: 10.1007/s10561-022-10018-4. Epub 2022 Jul 3.
One of the experimental programs for fertility protection in women includes protective cryopreservation. Vitroficasion of ovarian tissue is one of the protective cryopreservation methods that use high concentrations of antifreeze and faster cooling. To reduce its complications, LIF (Leukemia inhibitory factor) was used as a pretreatment in this study. In this study, the ovaries were randomly divided into 8 groups. In NCN (without pretreatment and LIF in culture media), NCP (without pretreatment and with LIF in culture media), PCP (with pretreatment and LIF in culture media), and PCN (with pretreatment and without LIF in culture media) groups, vitrification and reversal were not performed. In the groups NVN (without pretreatment and LIF in culture media), NVP (without pretreatment and with LIF in culture media) PV, PVP (with pretreatment and LIF in culture media), and PVN (with pretreatment and without LIF in culture medium) groups, vitrification and tissue reversal were performed. All groups were cultured and histological, cellular, and molecular evaluations were performed. The results of the present study showed that LIF in the culture medium reduced the number of abnormal, primordial, primary, and secondary follicles, and DNA breakage compared to the group without LIF (P < 0.05) and increases the growth of follicles and expression of GDF9, BMP, AMH, KITLG genes (P < 0.05). The use of LIF pretreatment before vitrification and melting of sheep ovary tissue in its culture medium reduces the damage caused by it and increases the growth and development of ovarian follicles while maintaining their function.
女性生育保护的实验方案之一包括保护性冷冻保存。卵巢组织玻璃化是保护性冷冻保存方法之一，该方法使用高浓度抗冻剂并采用更快的冷却速度。为减少其并发症，本研究使用白血病抑制因子（LIF）进行预处理。在本研究中，卵巢被随机分为8组。在NCN（培养基中无预处理和LIF）、NCP（培养基中无预处理但有LIF）、PCP（培养基中有预处理和LIF）和PCN（培养基中有预处理但无LIF）组中，未进行玻璃化和复温操作。在NVN（培养基中无预处理和LIF）、NVP（培养基中无预处理但有LIF）、PV、PVP（培养基中有预处理和LIF）和PVN（培养基中有预处理但无LIF）组中，进行了玻璃化和组织复温操作。所有组均进行培养，并进行组织学、细胞和分子评估。本研究结果表明，与无LIF组相比，培养基中的LIF减少了异常、原始、初级和次级卵泡的数量以及DNA断裂（P<0.05），并增加了卵泡生长以及生长分化因子9（GDF9）、骨形态发生蛋白（BMP）、抗苗勒管激素（AMH）、干细胞因子（KITLG）基因的表达（P<0.05）。在绵羊卵巢组织培养基中进行玻璃化和融化之前使用LIF预处理可减少其造成的损伤，并在维持卵巢卵泡功能的同时增加其生长和发育。
