Department of Gastroenterology, Panyu Central Hospital, Guangzhou, China.
Department of Clinical Laboratory, Panyu Central Hospital, Guangzhou, China.
Dis Markers. 2022 Jun 24;2022:7366337. doi: 10.1155/2022/7366337. eCollection 2022.
To explore the role of circIFITM1 and its potential molecular mechanism in colon cancer.
The circIFITM1 in human samples and cell lines of colon cancer was measured via RT-PCR. The cyclicity of circIFITM1 was confirmed by agarose gel electrophoresis and Sanger sequencing, and the stability of circIFITM1 was confirmed by actinomycin D assay. The proliferative and invasive ability was detected by the CCK-8 assay and Transwell assay, respectively. RNA pull-down assay confirmed a combination of circIFITM1 and miRNA. Dual-luciferase reporter gene was used to detect the direct relationship between miRNA and the target gene.
circIFITM1 originated from the maternal gene IFITM1and had high stability. It was resistant to processing by actinomycin D. Upregulating circIFITM1 facilitated the proliferation and invasion of Lovo cells, while interfering with circIFITM1 expression inhibited them. circIFITM1 interacted with miR-802, and miR-802 targeted the 3'UTR of FOXP1. The overexpression of circIFITM1 downregulated miR-802 and upregulated FOXP1.
circIFITM1 facilitates the proliferative and invasive abilities via miR-802/FOXP1 in Lovo cells.
探索 circIFITM1 在结肠癌中的作用及其潜在的分子机制。
采用 RT-PCR 检测人结肠癌组织和细胞系中的 circIFITM1。通过琼脂糖凝胶电泳和 Sanger 测序验证 circIFITM1 的环状结构，通过放线菌素 D 实验验证 circIFITM1 的稳定性。通过 CCK-8 检测和 Transwell 检测分别检测细胞的增殖和侵袭能力。RNA 下拉实验证实 circIFITM1 与 miRNA 结合。双荧光素酶报告基因检测用于检测 miRNA 与靶基因之间的直接关系。
circIFITM1 来源于母源基因 IFITM1，具有高度稳定性。它能抵抗放线菌素 D 的处理。上调 circIFITM1 促进了 Lovo 细胞的增殖和侵袭，而干扰 circIFITM1 表达则抑制了它们。circIFITM1 与 miR-802 相互作用，miR-802 靶向 FOXP1 的 3'UTR。circIFITM1 的过表达下调了 miR-802 并上调了 FOXP1。
circIFITM1 通过 miR-802/FOXP1 促进 Lovo 细胞的增殖和侵袭能力。
Zotero 插件
