Liu Zhao, Liu Qing, Xu Yanyan, Han Zhao, Zhang Ling, Li Xiaojing
Department of Dermatology, Affiliated Hospital of Hebei University of Engineering, Handan, China.
Department of Dermatology, Anqing Municipal Hospital, Anqing, China.
Front Microbiol. 2022 Jun 16;13:919677. doi: 10.3389/fmicb.2022.919677. eCollection 2022.
The purpose of this study was to investigate the effect and mechanism of 3% boric acid solution (BAS) against (CA) infection the interleukin-23 (IL-23)/T helper 17 cell (Th17) axis. 36 female mice were randomly divided into 3 groups, and 2 injection sites on the back of the mice were chosen at random. Group N was injected with sterile water for injection (SWFI), and Group M and Group B were injected with CA mycelium suspension. After successful model verification, the remaining mice entered the following treatments 5 days later. Group B was treated with 3% BAS, Group M was treated with SWFI, and Group N was not treated. Levels of interleukin-17 (IL-17), IL-22, and IL-23 in mouse blood were measured on days 1, 3, 5, and 7 of treatment. On day 7, IL-17, IL-22, and IL-23 in mouse skin were detected. Serum levels of IL-17, IL-22, and IL-23 in Group M were higher than in Group N on the first day of treatment ( < 0.05). Expression levels of IL-17, IL-22, and IL-23 in the epidermis of the skin lesions in Group M were higher than in Group N on day 7 ( < 0.05). The serum level of IL-17 in Group B was higher than in Group M on days 5 and 7 ( < 0.05). Serum levels of IL-22 in Group B on days 1, 5, and 7 were higher than in Group M ( < 0.05). Serum levels of IL-23 in Group B were higher than in Group M on days 3, 5, and 7 ( < 0.05). IL-17 and IL-23 in Group B reached a peak on day 5, significantly different on days 1, 3, and 7 ( < 0.05). The expression intensity of IL-17, IL-22, and IL-23 in the skin lesions of Group B was higher than that of Group M on day 7 ( < 0.05). We conclude that IL-17, IL-22, and IL-23 are involved in the anti-CA activity in mouse skin, and 3% BAS increased IL-17, IL-22, and IL-23 to mediate these effects.
本研究旨在探讨3%硼酸溶液（BAS）对白色念珠菌（CA）感染的影响及其作用机制，以及对白细胞介素-23（IL-23）/辅助性T细胞17（Th17）轴的影响。将36只雌性小鼠随机分为3组，在小鼠背部随机选择2个注射部位。N组注射注射用水（SWFI），M组和B组注射CA菌丝悬液。模型验证成功后，剩余小鼠于5天后进入以下处理。B组用3% BAS处理，M组用SWFI处理，N组不处理。在处理的第1、3、5和7天测量小鼠血液中白细胞介素-17（IL-17）、IL-22和IL-23的水平。在第7天，检测小鼠皮肤中的IL-17、IL-22和IL-23。处理第一天，M组血清中IL-17、IL-22和IL-23水平高于N组（<0.05）。第7天，M组皮肤病变表皮中IL-17、IL-22和IL-23的表达水平高于N组（<0.05）。B组第5天和第7天血清IL-17水平高于M组（<0.05）。B组第1、5和7天血清IL-22水平高于M组（<0.05）。B组第3、5和7天血清IL-23水平高于M组（<0.05）。B组IL-17和IL-23在第5天达到峰值，与第1、3和7天有显著差异（<0.05）。第7天，B组皮肤病变中IL-17、IL-22和IL-23的表达强度高于M组（<0.05）。我们得出结论，IL-17、IL-22和IL-23参与了小鼠皮肤抗CA的活性，3% BAS通过增加IL-17、IL-22和IL-23来介导这些作用。
