Yoshimatsu Masako, Kitaura Hideki, Morita Yukiko, Nakamura Takuya, Ukai Takashi
Oral Management Center, Nagasaki University Hospital, Nagasaki, Japan.
Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry, Sendai, Japan.
J Dent Sci. 2022 Jul;17(3):1087-1095. doi: 10.1016/j.jds.2022.02.007. Epub 2022 Feb 28.
BACKGROUND/PURPOSE: Orthodontic tooth movement is achieved by alveolar bone remodeling, and therefore the balance of bone resorption and formation is important. Receptor activator of nuclear factor-κB ligand (RANKL) plays a crucial role in bone resorption. We previously reported that tumor necrosis factor-α (TNF-α) is also important in bone resorption during tooth movement. In this study, we focused on bone and root resorption during orthodontic tooth movement in mice using anti-mouse RANKL antibody (anti-mRANKL ab).
Anti-mRANKL ab was administered intraperitoneally to mice that subsequently underwent orthodontic tooth movement. After 10 days, tissues around the moved teeth were histologically evaluated. To confirm the effects of anti-mRANKL ab on TNF-α induced bone resorption, TNF-α was administered with and without anti-mRANKL ab into the supracalvaria and the sutures of the calvaria were histologically evaluated.
Orthodontic tooth movement was suppressed in mice treated with anti-mRANKL ab. Root resorption was observed after orthodontic tooth movement, but not in mice treated with anti-mRANKL ab. In the calvarial experiment, the number of TRAP-positive cells in the calvarial sutures was lower in mice administered TNF-α with anti-mRANKL ab than in mice administered TNF-α alone.
Our findings suggest that anti-mRANKL ab suppressed orthodontic tooth movement. This needs to be considered when orthodontic tooth movement is required in patients using anti-RANKL antibody.
背景/目的：正畸牙齿移动是通过牙槽骨重塑实现的，因此骨吸收与形成的平衡很重要。核因子κB受体活化因子配体（RANKL）在骨吸收中起关键作用。我们之前报道过肿瘤坏死因子-α（TNF-α）在牙齿移动过程中的骨吸收中也很重要。在本研究中，我们使用抗小鼠RANKL抗体（抗-mRANKL抗体），重点研究了小鼠正畸牙齿移动过程中的骨和牙根吸收情况。
将抗-mRANKL抗体腹腔注射给随后进行正畸牙齿移动的小鼠。10天后，对移动牙齿周围的组织进行组织学评估。为了证实抗-mRANKL抗体对TNF-α诱导的骨吸收的影响，将TNF-α分别与抗-mRANKL抗体一起或不与抗-mRANKL抗体一起注射到颅顶骨上，并对颅顶骨缝线进行组织学评估。
用抗-mRANKL抗体处理的小鼠正畸牙齿移动受到抑制。正畸牙齿移动后观察到牙根吸收，但用抗-mRANKL抗体处理的小鼠未出现牙根吸收。在颅顶骨实验中，与单独注射TNF-α的小鼠相比，同时注射TNF-α和抗-mRANKL抗体的小鼠颅顶骨缝线中抗酒石酸酸性磷酸酶（TRAP）阳性细胞数量更少。
我们的研究结果表明抗-mRANKL抗体抑制了正畸牙齿移动。在使用抗RANKL抗体的患者需要进行正畸牙齿移动时，这一点需要考虑。
