SNHG5/miR-299-5p/ATF2 轴作为椎间盘退变免疫微环境的生物标志物。

SNHG5/miR-299-5p/ATF2 Axis as a Biomarker in Immune Microenvironment of Intervertebral Disc Degeneration.

机构信息

Department of Rehabilitation, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.

Department of Rehabilitation, Guangzhou Red Cross Hospital, Jinan University, Guangzhou 510000, China.

出版信息

Mediators Inflamm. 2022 Jun 23;2022:2558275. doi: 10.1155/2022/2558275. eCollection 2022.

METHODS

The data sets of GSE56081 and GSE63492 in the Gene Expression Omnibus (GEO) database were used for screening and analysis, and the key gene markers were verified by GSE34095 and GSE126883. Finally, the infiltration of immune cells in the data were analyzed by MCPcounter analysis package.

RESULTS

In this study, a ceRNA containing 15 lncRNAs, 9 miRNAs, and 103 mRNAs was constructed. After multimodel screening and verification, key gene marker was found, namely, ATF2. The lncRNA/miRNA/mRNA axis closely related to ATF2 have also been found, namely, SNHG5/miR-299-5p/ATF2. In the analysis of immune infiltration, ATF2 was negatively correlated with T cells but positively correlated with neutrophils and endothelial cells.

CONCLUSION

The SNHG5/miR-299-5p/ATF2 can be used as biomarker of IDD, and infiltration of immune cells plays an important role in the pathological development of IDD. In addition, as a marker of IDD, the involvement of the above-mentioned axis in the pathological development of IDD remains to be further explored.

方法

本研究从基因表达综合数据库（GEO）中筛选并分析了数据集 GSE56081 和 GSE63492，利用 GSE34095 和 GSE126883 对关键基因标志物进行了验证，最后通过 MCPcounter 分析程序包分析了数据中免疫细胞的浸润情况。

结果

本研究构建了一个包含 15 个 lncRNA、9 个 miRNA 和 103 个 mRNA 的 ceRNA。经过多模型筛选和验证，发现关键基因标志物为 ATF2。还发现了与 ATF2 密切相关的 lncRNA/miRNA/mRNA 轴，即 SNHG5/miR-299-5p/ATF2。在免疫细胞浸润分析中，ATF2 与 T 细胞呈负相关，与中性粒细胞和内皮细胞呈正相关。