Key Lab of Plant Biotechnology in University of Shandong Province, College of Life Science, Qingdao Agricultural University, Qingdao, China.
Key Lab of Plant Biotechnology in University of Shandong Province, College of Life Science, Qingdao Agricultural University, Qingdao, China.
Plant Sci. 2022 Oct;323:111370. doi: 10.1016/j.plantsci.2022.111370. Epub 2022 Jul 2.
With the intensification of global warming, extreme weather events have occurred more frequently, among which cold stress has become one of the major environmental factors that restrict global crop yield and production. Multiple long noncoding RNAs (lncRNAs) have been predicted or recognized in the plant response to cold stress, however, the molecular biological functions of most of these RNAs are still poorly understood. Here, we identified a novel lncRNA, COLD INDUCED lncRNA 1 (CIL1), as a positive regulator of the plant response to cold stress in Arabidopsis. CIL1 was significantly induced when the plant was exposed to cold stress. Moreover, knockdown mutants showed more sensitivity to cold stress than the wild type did, accompanied by an increased content of endogenous ROS (reactive oxygen species) and reduced osmoregulatory substances. Genome-wide transcriptome analysis indicated that 256 genes were downregulated and 34 genes were upregulated in cil1 mutants under cold stress, which were mainly involved in hormone signal transduction, ROS homeostasis and glucose metabolism. Our study implies that CIL1 has a positive effect on the plant response to cold stress by regulating the expression of multiple stress-related genes during the seedling stage.
随着全球变暖的加剧，极端天气事件发生得更加频繁，其中冷应激已成为限制全球作物产量和生产的主要环境因素之一。在植物对冷应激的反应中，已经预测或识别出了多个长非编码 RNA（lncRNA），然而，这些 RNA 的大多数分子生物学功能仍知之甚少。在这里，我们鉴定了一个新的 lncRNA，即冷诱导 lncRNA 1（CIL1），它作为拟南芥对冷应激反应的正调节剂。当植物受到冷应激时，CIL1 明显被诱导。此外，与野生型相比，敲低突变体对冷应激更为敏感，伴随着内源性 ROS（活性氧）含量增加和渗透调节物质减少。全基因组转录组分析表明，cil1 突变体在冷胁迫下有 256 个基因下调和 34 个基因上调，这些基因主要参与激素信号转导、ROS 稳态和葡萄糖代谢。我们的研究表明，CIL1 通过在幼苗期调节多个与应激相关基因的表达，对植物的冷应激反应具有积极作用。
