Department of Oral and Maxillofacial Surgery, the Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266000, PR China.
Department of General Dentistry and Emergency, School of Stomatology, Air Force Military Medical University, Xi'an, Shanxi, 710032, PR China.
Bull Cancer. 2022 Sep;109(9):886-894. doi: 10.1016/j.bulcan.2022.05.004. Epub 2022 Jul 1.
Epithelial-mesenchymal transition (EMT) is a key initial step in the recurrence and metastasis of tongue squamous cell carcinoma (TSCC). Hyperthermia (HT) may reduce the rate of postoperative recurrence and distant metastasis by reversing the process of EMT of tumor cells, but the molecular mechanism is unclear. This study aims to investigate the role of inhibitor of differentiation/DNA binding-1 (Id-1) in HT mediated reversal of EMT of TSCC cells, and to provide a new approach for the treatment of TSCC using therapeutic gene targeting. After the combination of RNA interference with Id-1 and HT, the morphology of TSCC cells changed from spindle-like to pebble-like, and the arrangement of cells changed from loose and disorderly to compact and orderly. The silencing of Id-1 gene enhances the efficacy of HT by affecting the expression of EMT markers in TSCC cells. This study suggests that the Id-1 gene in TSCC cells can regulate transforming growth factor-beta 1, thereby affecting the expression of EMT markers, to achieve the effect of reducing HT.
上皮-间充质转化（EMT）是舌鳞状细胞癌（TSCC）复发和转移的关键初始步骤。热疗（HT）可能通过逆转肿瘤细胞的 EMT 过程来降低术后复发和远处转移的速度，但分子机制尚不清楚。本研究旨在探讨分化/DNA 结合抑制因子-1（Id-1）在 HT 介导的 TSCC 细胞 EMT 逆转中的作用，并为采用治疗性基因靶向治疗 TSCC 提供新的方法。在 RNA 干扰与 Id-1 结合并进行 HT 后，TSCC 细胞的形态从纺锤形变为鹅卵石形，细胞排列从疏松紊乱变为紧密有序。Id-1 基因的沉默通过影响 TSCC 细胞中 EMT 标志物的表达增强了 HT 的疗效。本研究表明，TSCC 细胞中的 Id-1 基因可以调节转化生长因子-β1，从而影响 EMT 标志物的表达，以达到降低 HT 的效果。
