绿原酸抑制食管鳞癌细胞转移的机制：EGFR/p-Akt/Snail 信号通路。
Chlorogenic Acid Inhibition of Esophageal Squamous Cell Carcinoma Metastasis EGFR/p-Akt/Snail Signaling Pathways.
机构信息
Department of Food Science and Nutrition, Meiho University, Pingtung, Taiwan, R.O.C.
Department of Biochemistry, School of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, R.O.C.
出版信息
Anticancer Res. 2022 Jul;42(7):3389-3402. doi: 10.21873/anticanres.15826.
BACKGROUND/AIM: Chlorogenic acid (CGA) is a polyphenol compound found in a variety of foods, including coffee, tea, cherries, and apples. It has been found by a number of studies to affect the viability of human cancer cells. No study has investigated its effect on esophageal squamous cell carcinoma (ESCC) metastasis or the molecular mechanism underlying its effect on this disease.
MATERIALS AND METHODS
We first used the Taiwanese ESCC cell line CE81T/VGH to create CE81T-M4 cells. Treatment of higher motility cells with chlorogenic acid for 24 h led to inhibition of cell migration and invasion as shown by scratch migration and transwell assays.
RESULTS
Western blotting showed that chlorogenic acid halted the activation of EGFR/p-Akt/Snail pathway and suppressed the expression of MMP-2 and MMP-9. Knockdown of either EGFR or Akt inhibited Snail, MMP2, and MMP9 activity as well as cell migration and invasion.
CONCLUSION
Chlorogenic acid inhibited cancer cell motility via the EGFR/p-Akt/Snail pathway and could potentially be used to develop an antimetastatic agent for ESCC in the future.
背景/目的：绿原酸（CGA）是一种存在于多种食物中的多酚化合物，包括咖啡、茶、樱桃和苹果。多项研究发现它会影响人类癌细胞的活力。目前尚无研究调查其对食管鳞状细胞癌（ESCC）转移的影响，也没有研究调查其对该疾病影响的分子机制。
材料与方法
我们首先使用台湾 ESCC 细胞系 CE81T/VGH 构建了 CE81T-M4 细胞。划痕迁移和 Transwell 分析显示，用绿原酸处理高迁移能力的细胞 24 小时后，可抑制细胞迁移和侵袭。
结果
Western blot 分析显示，绿原酸阻断了 EGFR/p-Akt/Snail 通路的激活，并抑制了 MMP-2 和 MMP-9 的表达。EGFR 或 Akt 的敲低均抑制了 Snail、MMP2 和 MMP9 的活性以及细胞迁移和侵袭。
结论
绿原酸通过 EGFR/p-Akt/Snail 通路抑制癌细胞迁移，未来可能用于开发 ESCC 的抗转移药物。
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