Department of Biochemistry, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Turkey.
Department of Chemistry, Faculty of Science, Van Yuzuncu Yil University, Van, Turkey.
Pak J Pharm Sci. 2022 May;35(3):801-805.
Angiotensin converting enzyme (ACE, EC 3.4.15.1) is an important enzyme responsible for regulating blood pressure. Inhibition of this enzyme is an important treatment approach in the treatment of hypertension, and natural or synthetic ACE inhibitors are often used for this purpose. In this study, the preventive effect of two important antioxidant compounds, lycopene (LYC) and thymoquinone (TQ) on ACE activity in human plasma was investigated. Human plasma was used as ACE source. ACE activity was calculated absorbance at 345 nm after incubation for 30 minutes at 35°C. TQ and LYC showed inhibitory effect on ACE activity. IC values for TQ and LYC were determined as 314μM and 182 μM, respectively. Type of inhibition for TQ and lycopene from plot Line weaver-Burk was designated as non-competitive inhibition. The Ki constants of TQ and LYC were determined to be 707 μM and 167 μM, respectively. It was concluded that TQ and LYC may have significant potential as ACE inhibitors.
血管紧张素转换酶（ACE，EC 3.4.15.1）是一种重要的酶，负责调节血压。抑制这种酶是治疗高血压的重要治疗方法，通常使用天然或合成的 ACE 抑制剂来达到此目的。在这项研究中，研究了两种重要抗氧化化合物番茄红素（LYC）和百里醌（TQ）对人血浆中 ACE 活性的预防作用。用人血浆作为 ACE 来源。在 35°C 孵育 30 分钟后，通过吸光度在 345nm 处计算 ACE 活性。TQ 和 LYC 对 ACE 活性表现出抑制作用。TQ 和 LYC 的 IC 值分别确定为 314μM 和 182μM。TQ 和番茄红素的抑制类型根据 Line weaver-Burk 作图指定为非竞争性抑制。TQ 和 LYC 的 Ki 常数分别确定为 707μM 和 167μM。结论是，TQ 和 LYC 可能具有作为 ACE 抑制剂的显著潜力。
