de Oliveira Pedro Henrique Chaves, Gomes Filho João Eduardo, Rodrigues Michael Júnio da Silva, da Silva Cristiane Cantiga, Cardoso Carolina da Barros Moraes, Cosme daSilva Leopoldo, Ervolino Edilson, Cintra Luciano Tavares Angelo
Endodontic Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (Unesp), Araçatuba, Brazil.
Department of Endodontics, School of Dentistry, Alagoas Federal University (UFAL), Maceió, Brazil.
Int Endod J. 2022 Oct;55(10):1026-1041. doi: 10.1111/iej.13795. Epub 2022 Jul 16.
Natural substances such as omega-3 have been used in the medical field due to their numerous properties and, in particular, modulating effect on the systemic and local inflammatory processes. Thus, this study evaluated the influence of omega-3 supplementation on the subcutaneous tissue response of endodontic sealers in Wistar Rats.
Polyethylene tubes were implanted in the subcutaneous tissue of 48 animals (one empty for control and three filled with Sealapex, AH Plus or Endofill). The animals were treated with omega-3 (TO) or water (TW). Treatments started 15 days before implantation until euthanasia. After 5, 15 and 30 days (n = 8), animals were euthanized and polyethylene tubes and surrounding tissue were removed and processed for histological analysis. The inflammatory reaction was analysed by Haematoxylin and Eosin stain and immunolabelling for IL-6 and TNF-α. The collagen maturity was analysed by picrosirius red stain and calcium deposition by von Kossa stain and polarized light. Results were statistically analysed (p < .05).
Amongst TW sealer groups, Endofill evoked a more intense inflammatory infiltrate compared with AH Plus and control in the 30-day period (p = .009). However, in TO sealer groups, there was no difference amongst the sealers and control in all periods (p > .05). Comparing each sealer as a function of the supplementation with water or omega-3, there are differences for Endofill (p = .001) and Sealapex (p = .005) in the 30-day period, presenting lower inflammatory infiltrate in the animals treated with omega-3. A higher percentage of immature fibres was observed at 15 and 30 days in the TO group, compared with the TW group (p < .05). The deposition of calcium particles was observed only by Sealapex in all periods, despite the supplementation procedure.
Omega-3 supplementation influence the tissue reactions of endodontic sealers, modulating inflammation, the immunolabelling of IL-6 and TNF-α, the repair process and it does not interfere with calcium deposition.
ω-3等天然物质因其多种特性，尤其是对全身和局部炎症过程的调节作用，已被应用于医学领域。因此，本研究评估了补充ω-3对Wistar大鼠牙髓封闭剂皮下组织反应的影响。
将聚乙烯管植入48只动物的皮下组织（1个空管作为对照，3个分别填充Sealapex、AH Plus或Endofill）。动物分别接受ω-3（TO组）或水（TW组）处理。处理从植入前15天开始直至安乐死。在5天、15天和30天后（每组n = 8），对动物实施安乐死，并取出聚乙烯管及周围组织进行组织学分析。通过苏木精和伊红染色分析炎症反应，并对白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）进行免疫标记。通过天狼星红染色分析胶原成熟度，通过冯·科萨染色和偏振光分析钙沉积情况。对结果进行统计学分析（p < 0.05）。
在TW封闭剂组中，与AH Plus和对照组相比，Endofill在30天时引起了更强烈的炎症浸润（p = 0.009）。然而，在TO封闭剂组中，各封闭剂组与对照组在所有时间段均无差异（p > 0.05）。将每种封闭剂作为水或ω-3补充的函数进行比较，在30天时Endofill（p = 0.001）和Sealapex（p = 0.005）存在差异，ω-3处理的动物炎症浸润较低。与TW组相比，TO组在15天和30天时观察到更高比例的未成熟纤维（p < 0.05）。尽管有补充程序，但在所有时间段仅观察到Sealapex有钙颗粒沉积。
补充ω-3会影响牙髓封闭剂的组织反应，调节炎症、IL-6和TNF-α的免疫标记以及修复过程，并且不干扰钙沉积。
