Torres-Ruiz J, McFadden B A
Arch Biochem Biophys. 1987 Apr;254(1):63-8. doi: 10.1016/0003-9861(87)90081-6.
L8 and L8S8 forms of ribulose bisphosphate carboxylase/oxygenase (RubisCO) have been prepared from Chromatium vinosum by the extremely mild method of centrifugal fractionation. Only the L8S8 form is detectable in crude extracts of this organism. Both forms show immunological identify in double diffusion studies using antibody to L subunits of the L8S8 form. L subunits from both L8 and L8S8 enzymes are identical by the criteria of peptides observed after limited proteolysis and N-terminal sequence analysis. In addition, these subunits show regions of homology with L subunits from Rhodospirillum rubrum, Anacystis nidulans, and spinach. S subunits of the C. vinosum enzyme are completely homologous to those from A. nidulans and higher plants from the 18th through 25th residue, a stretch preceded in all cases by two basic amino acids.
已通过极其温和的离心分级分离方法从嗜硫红假单胞菌中制备出核糖二磷酸羧化酶/加氧酶（RubisCO）的L8和L8S8形式。在该生物体的粗提物中仅可检测到L8S8形式。在使用针对L8S8形式的L亚基的抗体进行的双向扩散研究中，两种形式均显示出免疫同一性。根据有限蛋白酶解后观察到的肽段和N端序列分析标准，L8和L8S8酶的L亚基是相同的。此外，这些亚基与来自深红红螺菌、集胞藻和菠菜的L亚基显示出同源区域。嗜硫红假单胞菌酶的S亚基与来自集胞藻和高等植物的S亚基在第18至25个残基处完全同源，在所有情况下，这一区域之前都有两个碱性氨基酸。
