College of Animal Science, TarimUniversity, Alar, China.
Xinjiang Urumqi Animal Husbandry and Veterinary General Station, Urumqi, China.
DNA Cell Biol. 2022 Jul;41(7):643-656. doi: 10.1089/dna.2021.1018.
The aim of this study was to study the relationship between the methylation level of the promoter region of follicle-stimulating hormone receptor () gene and the mRNA expression of Duolang sheep fed different energy diets. In this experiment, three groups of diets with different energy levels (dietary nutrient level reference (NY/T816 - 2004)) were designed to obtain medium energy level diets with metabolic energy of 10.88 MJ/d. The treatments with high and low energy levels increased and decreased by 15%, 12.51, and 9.25 MJ/d, respectively, on the basis of medium energy level. Total RNA and genomic DNA were extracted from the ovaries of Duolang sheep, and qPCR was performed. Bisulfite genomic sequencing PCR and sequence alignment were used to detect the relationship between the relative expression level of FSHR and methylation. The results showed that the expression of FSHR in high-energy group and medium-energy group was significantly higher than that in low-energy group ( < 0.01), and there was no significant difference between high-energy group and medium-energy group ( > 0.05). The methylation rate of the target fragment in the promoter region of FSHR gene was 41.67% in the high-energy group, 75.00% in the medium-energy group, and 83.33% in the low-energy group. This study revealed that different dietary energy levels had certain effects on DNA methylation level of FSHR gene and then affected gene expression, and the expression level was negatively correlated with methylation level.
本研究旨在研究促卵泡激素受体（FSHR）基因启动子区域甲基化水平与不同能量日粮喂养的都兰羊 mRNA 表达之间的关系。在本实验中，设计了三组不同能量水平的日粮（饲粮营养水平参考（NY/T816-2004）），以获得代谢能为 10.88MJ/d 的中等能量水平日粮。高能和低能水平的处理分别在中能水平的基础上增加和减少 15%、12.51 和 9.25MJ/d。从都兰羊的卵巢中提取总 RNA 和基因组 DNA，并进行 qPCR。亚硫酸氢盐基因组测序 PCR 和序列比对用于检测 FSHR 相对表达水平与甲基化之间的关系。结果表明，高能组和中能组 FSHR 的表达显著高于低能组（<0.01），而高能组和中能组之间无显著差异（>0.05）。FSHR 基因启动子区域目标片段的甲基化率在高能组为 41.67%，中能组为 75.00%，低能组为 83.33%。本研究表明，不同的日粮能量水平对 FSHR 基因的 DNA 甲基化水平有一定影响，进而影响基因表达，且表达水平与甲基化水平呈负相关。
