College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210046, China.
College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210046, China.
Phytomedicine. 2022 Sep;104:154304. doi: 10.1016/j.phymed.2022.154304. Epub 2022 Jun 30.
Oxidative stress played a key role in the development of bone brittleness and is an important pathogenic factor of senile osteoporosis. A variety of animal and plant-derived peptides have been shown to have significant anti-osteoporosis effects in vivo and in vitro.
In this study, we aim to explore the possible mechanism of wheat germ peptide ADWGGPLPH on senile osteoporosis.
Naturally, aged rats were used as animal models of senile osteoporosis.
Wheat germ peptide ADWGGPLPH was administered from 9-months-old to 21-months-old, and the effect of ADWGGPLPH on preventing senile osteoporosis was evaluated by measuring serum biochemical indexes, bone histomorphometry, bone biomechanics, and other indexes to elucidate the mechanism of ADWGGPLPH in delaying senile osteoporosis by detecting the expression of osteoporosis-related proteins.
The results showed that ADWGGPLPH could effectively reduce the level of oxidative stress and improve the microstructure and bone mineral density in senile osteoporosis rats. In addition, ADWGGPLPH could improve the proliferation and differentiation activity of osteoblasts and effectively inhibit osteoclasts' differentiation by regulating the OPG/RANKL/RANK/TRAF6 pathway.
ADWGGPLPH from wheat germ exhibited a notably effect on senile osteoporosis and has a high potential in the development of the nutrient regimen to against senile osteoporosis.
氧化应激在骨脆性的发展中起关键作用，是老年性骨质疏松症的重要致病因素。多种动植物来源的肽已被证明在体内和体外具有显著的抗骨质疏松作用。
本研究旨在探讨麦胚肽 ADWGGPLPH 对老年性骨质疏松症的可能作用机制。
自然地，老年大鼠被用作老年性骨质疏松症的动物模型。
从 9 月龄到 21 月龄给予麦胚肽 ADWGGPLPH，通过测量血清生化指标、骨组织形态计量学、骨生物力学等指标来评估 ADWGGPLPH 对预防老年性骨质疏松症的效果，以阐明 ADWGGPLPH 通过检测与骨质疏松症相关蛋白的表达来延缓老年性骨质疏松症的机制。
结果表明，ADWGGPLPH 可有效降低氧化应激水平，改善老年性骨质疏松症大鼠的骨微结构和骨密度。此外，ADWGGPLPH 可通过调节 OPG/RANKL/RANK/TRAF6 通路来提高成骨细胞的增殖和分化活性，并有效抑制破骨细胞的分化。
麦胚 ADWGGPLPH 对老年性骨质疏松症具有显著的防治作用，在开发防治老年性骨质疏松症的营养方案方面具有很高的潜力。
