Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.
Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.
J Glob Antimicrob Resist. 2022 Sep;30:237-240. doi: 10.1016/j.jgar.2022.06.028. Epub 2022 Jul 3.
Multidrug-resistant (MDR) clones of Pseudomonas aeruginosa can cause complicated infections in human. The emergence of ST664 of MDR P. aeruginosa has been reported in Nepal, Iran and China. Here, we present the draft genome analysis of a MDR P. aeruginosa CMPL223 isolated from hospital wastewater in Bangladesh to understand antimicrobial resistance trends and pathogenicity.
Cetrimide agar was used for isolation of P. aeruginosa. Polymerase chain reaction (PCR) was carried out for detection of biofilm and integron related genes. Bacterial susceptibility to antibiotics was determined by disc diffusion method. Sequencing of whole genomic DNA was performed using Illumina iSeq 100 platform. Following quality checking of raw reads, assembly and annotation of sequences, a wide array of in silico tools were used for characterization of draft genome.
The isolate was a strong biofilm former, carried integron 1 in chromosomal DNA, and was predicted to be pathogenic. It belongs to sequence type ST664 and O7 serogroup. The assembled genome contained 12 acquired antimicrobial resistant (AMR) genes, 2 prophage regions, 240 virulence genes, 71 drug targets, 142 insertion sequences, and 1 CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) array. The isolate was resistant to 21 out of 23 antibiotics, except colistin and imipenem. Comprehensive Antibiotic Resistance Database and ResFinder revealed that bacteria harboured bla, bla, bla fosA, tet(G), sul1, catB7, aph(3')-iib and ant(4')-IIb genes, conferring resistance to different classes of antibiotics. The results of in vitro characterization were consistent with the possible expression of detected antibiotic resistant genes through in silico analysis.
Our data suggested the emergence of MDR P. aeruginosa ST664, which needs control measures for limiting its dissemination.
铜绿假单胞菌（Pseudomonas aeruginosa）的多药耐药（MDR）克隆可导致人类发生复杂感染。在尼泊尔、伊朗和中国已经报道了 MDR 铜绿假单胞菌 ST664 的出现。在此，我们对从孟加拉国医院废水中分离出的一株 MDR 铜绿假单胞菌 CMPL223 进行了基因组草案分析，以了解其抗药性趋势和致病性。
采用 Cetrimide 琼脂分离铜绿假单胞菌。采用聚合酶链反应（PCR）检测生物膜和整合子相关基因。采用纸片扩散法测定细菌对抗生素的敏感性。采用 Illumina iSeq 100 平台对全基因组 DNA 进行测序。对原始读数进行质量检查后，对序列进行组装和注释，然后使用多种基于计算机的工具对基因组草案进行特征描述。
该分离株是一种强生物膜形成菌，在染色体 DNA 中携带整合子 1，且被预测为致病性的。它属于序列类型 ST664 和 O7 血清群。组装的基因组包含 12 种获得性抗微生物药物耐药（AMR）基因、2 个噬菌体区域、240 个毒力基因、71 个药物靶点、142 个插入序列和 1 个 CRISPR（簇状规则间隔短回文重复）阵列。该分离株对 23 种抗生素中的 21 种耐药，除了多粘菌素和亚胺培南。综合抗生素耐药数据库和 ResFinder 显示，细菌携带 bla、bla、bla fosA、tet(G)、sul1、catB7、aph(3')-iib 和 ant(4')-IIb 基因，对不同类别的抗生素产生耐药性。体外特性的结果与通过计算机分析可能表达的检测到的抗生素耐药基因的结果一致。
我们的数据表明，MDR 铜绿假单胞菌 ST664 的出现需要采取控制措施来限制其传播。
