Department of chemistry, Indian Institute of Technology Jodhpur, Jodhpur, 342037, Rajasthan, India.
Chemphyschem. 2022 Sep 16;23(18):e202200265. doi: 10.1002/cphc.202200265. Epub 2022 Jul 7.
Clinical trials on the therapeutic effect of curcumin have proven to be highly effective against many diseases including cancer and Alzheimer's. However, the molecular mechanism of interaction of curcumin with protein and live cell membrane is poorly understood. Here, we report the mechanism of interaction of curcumin with bovine serum albumin (BSA) and live E. coli cell membrane in the presence of organized assemblies of sodium dodecyl sulfate (SDS) and cetrimonium bromide (CTAB) by fluorescence spectroscopy, laser-scanning confocal microscopy, and computation. Enhanced binding constant, blue-shifted emission spectra, and imaging of heterogeneous FRET on live bacteria cell membrane strongly indicate the complex formation of curcumin with strong hydrophobic interaction, which is further validated by computation. Finally, our results may shed light on the efficient strategy of applications of curcumin as a natural therapeutic lead in clinical trials against many life-threatening diseases.
临床试验已经证明姜黄素在治疗许多疾病方面非常有效，包括癌症和阿尔茨海默病。然而，姜黄素与蛋白质和活细胞膜相互作用的分子机制还知之甚少。在这里，我们通过荧光光谱、激光扫描共聚焦显微镜和计算报告了姜黄素与牛血清白蛋白（BSA）和活大肠杆菌细胞膜在有序组装的十二烷基硫酸钠（SDS）和十六烷基三甲基溴化铵（CTAB）存在下相互作用的机制。增强的结合常数、发射光谱的蓝移以及对活细菌细胞膜上不均匀的 FRET 的成像强烈表明姜黄素与强疏水相互作用形成复合物，这通过计算进一步得到了验证。最后，我们的结果可能为姜黄素作为一种天然治疗先导物在临床试验中治疗许多危及生命的疾病的有效应用策略提供了启示。
