Invertebrate Genetics Laboratory, Department of Chromosome Science, National Institute of Genetics, Research Organization of Information and Systems (ROIS), Mishima, Shizuoka, Japan.
Division of Invertebrate Genetics, Department of Genetics, The Graduate University for Advanced Studies (SOKENDAI), Mishima, Shizuoka, Japan.
Methods Mol Biol. 2022;2509:135-141. doi: 10.1007/978-1-0716-2380-0_8.
Emerging evidence indicates that PIWI proteins, in collaboration with PIWI-interacting RNAs (piRNAs), play a critical role in gonadal development and retrotransposon silencing in metazoans. Numerous studies have characterized the mechanism of retrotransposon silencing and identified dozens of factors involved in the piRNA pathways. Drosophila is an attractive model organism for piRNA studies due to its great availability of genetic tools and the low cost of maintenance. Here, I introduce Drosophila genetic resources and techniques valuable for studying piRNA pathway genes via their impact on retrotransposon silencing.
新出现的证据表明，PIWI 蛋白与 PIWI 相互作用 RNA（piRNA）一起，在后生动物的性腺发育和逆转座子沉默中发挥着关键作用。许多研究已经描述了逆转座子沉默的机制，并鉴定了数十种参与 piRNA 途径的因子。果蝇是研究 piRNA 的理想模式生物，因为它具有丰富的遗传工具和低廉的维护成本。在这里，我介绍了通过其对逆转座子沉默的影响来研究 piRNA 通路基因的果蝇遗传资源和技术。
