Warton A, Papadimitriou J M, Goldie R G, Paterson J W
Aust J Exp Biol Med Sci. 1986 Oct;64 ( Pt 5):435-44. doi: 10.1038/icb.1986.46.
Specimens of normal and asthmatic lungs were studied at the electron microscopic level and the frequency and ultrastructural features of mast cells and their granules within the alveolar wall were assessed with morphometric techniques. The numerical density of mast cells per square millimetre of alveolar wall was 299 (SD = 258) in normal and 366 (SD = 260) in asthmatic lung. The mean area of the mast cell nucleated profile was 25.7 microns2 (SD = 6.3) in normal lung and 29.8 (SD = 6.2) in asthmatic. The average number of secretory granules per single mast cell nucleated profile was 55 (SD = 13) in normal lung and 60 (SD = 12) in asthmatic lung. The diameter of the individual secretory granule was 338.9 nm (SD = 42.6) in normal and 345.6 (SD = 47.7) in asthmatic lung. The volume density of secretory granules in normal and asthmatic lung was 6.31 microns3 and 5.81 microns3, respectively. The mean diameter of the individual subunit ('scroll') inside the secretory granule was 88.8 nm for both normal and asthmatic lung. In normal lung 64.2% of granules were of 'scroll' and 'combined' type, and 35.8% of granules were 'particulate' or 'empty'. In specimens from asthmatic patients 40.3% of granules had 'scroll' or 'combined' structures and 59.7% were 'particulate' or 'empty'. Our data suggest that there is no difference between the number of mast cells in normal and asthmatic lung. However, in pulmonary mast cells from asthmatic lung, degranulation is more common than in normal lung.
在电子显微镜水平下研究了正常肺和哮喘肺的标本，并采用形态计量学技术评估了肺泡壁内肥大细胞及其颗粒的频率和超微结构特征。正常肺每平方毫米肺泡壁内肥大细胞的数量密度为299（标准差=258），哮喘肺为366（标准差=260）。正常肺中肥大细胞核轮廓的平均面积为25.7平方微米（标准差=6.3），哮喘肺中为29.8（标准差=6.2）。正常肺中单个肥大细胞核轮廓的分泌颗粒平均数量为55（标准差=13），哮喘肺中为60（标准差=12）。正常肺中单个分泌颗粒的直径为338.9纳米（标准差=42.6），哮喘肺中为345.6（标准差=47.7）。正常肺和哮喘肺中分泌颗粒的体积密度分别为6.31立方微米和5.81立方微米。正常肺和哮喘肺中分泌颗粒内单个亚单位（“卷轴”）的平均直径均为88.8纳米。正常肺中64.2%的颗粒为“卷轴”型和“组合”型，35.8%的颗粒为“颗粒”型或“空泡”型。哮喘患者标本中40.3%的颗粒具有“卷轴”或“组合”结构，59.7%为“颗粒”型或“空泡”型。我们的数据表明，正常肺和哮喘肺中肥大细胞的数量没有差异。然而，在哮喘肺的肺肥大细胞中，脱颗粒比正常肺更常见。
