Department of Biomedical Engineering, State University of New York at Binghamton, Binghamton, New York 13902, United States.
Decker College of Nursing and Health Sciences, State University of New York at Binghamton, Johnson City, New York 13790, United States.
ACS Sens. 2022 Jul 22;7(7):1996-2005. doi: 10.1021/acssensors.2c00787. Epub 2022 Jul 7.
The wound healing process remains a poorly understood biological mechanism. The high morbidity and mortality rates associated with chronic wounds are a critical concern to the health care industry. Although assessments and treatment options exist, these strategies have primarily relied on static wound dressings that do not consider the dynamic physicochemical microenvironment and can often create additional complications through the frequent dressing changing procedure. Inspired by the need for engineering "smart" bandages, this study resulted in a multifaceted approach to developing an adhesive-free, permeable, and multiplex sensor system. The electronic-extracellular matrix (e-ECM) platform is capable of noninvasively monitoring chemical and physical changes in real-time on a flexible, stretchable, and permeable biointegrated platform. The multiplex sensors are constructed atop a soft, thin, and microfibrous substrate of silicone to yield a conformal, adhesive-free, convective, or diffusive wound exudate flow, and passive gas transfer for increased cellular epithelization and unobstructed physical and chemical sensor monitoring at the wound site. This platform emulates the native epidermal mechanics and physical extracellular matrix architecture for intimate bio-integration. The multiple biosensor array can continuously examine inflammatory biomarker such as lactate, glucose, pH, oxygen, and wound temperature that correlates to the wound healing status. Additionally, a heating element was incorporated to maintain the optimal thermal conditions at the wound bed. The e-ECM electrochemical biosensors were tested in vitro, within phosphate-buffered saline, and ex vivo, within wound exudate. The "smart" wound bandage combines biocompatible materials, treatments, and monitoring modalities on a microfibrous platform for complex wound dynamic control and analysis.
伤口愈合过程仍然是一个了解甚少的生物学机制。慢性伤口相关的高发病率和死亡率是医疗保健行业的一个关键关注点。尽管存在评估和治疗选择，但这些策略主要依赖于不考虑动态物理化学微环境的静态伤口敷料，并且经常通过频繁的敷料更换程序造成额外的并发症。受需要开发“智能”绷带的启发，本研究提出了一种多方面的方法来开发一种无粘性、可渗透和多功能传感器系统。电子细胞外基质（e-ECM）平台能够在灵活、可拉伸和可渗透的生物集成平台上非侵入性地实时监测化学和物理变化。多个传感器构建在柔软、薄和微纤维硅基质上，以产生顺应性、无粘性、对流或扩散的伤口渗出物流动以及被动气体转移，从而促进细胞上皮化，并在伤口部位实现无阻碍的物理和化学传感器监测。该平台模拟了天然表皮力学和物理细胞外基质结构，实现了亲密的生物集成。多个生物传感器阵列可以连续检查炎症生物标志物，如乳酸盐、葡萄糖、pH 值、氧气和伤口温度，这些标志物与伤口愈合状态相关。此外，还加入了一个加热元件，以维持伤口床的最佳热条件。e-ECM 电化学生物传感器在磷酸盐缓冲盐溶液中和体外伤口渗出液中进行了测试。“智能”伤口绷带将生物相容性材料、治疗方法和监测模式结合在微纤维平台上，用于复杂的伤口动态控制和分析。
