College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China.
Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou 515063, China.
J Proteomics. 2022 Aug 30;266:104668. doi: 10.1016/j.jprot.2022.104668. Epub 2022 Jul 4.
The hemostatic effect of isinglass (dried swim bladder) in traditional Chinese medicine is well known. But its mechanism of action remains unclear. Here, mice were gavaged with the dried swim bladder of the chu's croaker (Nibea coibor). The hemostatic effect of swim bladder was investigated, tandem mass tag (TMT)-based quantitative proteomics analysis was performed to screen differentially abundant proteins associated with hemostasis in mouse serum. Results indicated that isinglass significantly shorten bleeding time and promoted coagulation after acute trauma (cut out mouse tail). In total, 57 differentially expressed proteins were identified in the sera between control and swim bladder group, of which 31 were up-regulated and 26 were down-regulated in swim bladder group. KEGG pathway enrichment analysis further demonstrated that the Neutrophil extracellular trap formation pathway was significantly affected. Combined with RT-qPCR verification, our findings further suggested that five candidate proteins in the pathway may be involved in the onset of hemostasis after swim bladder gavage, indicating their important role during the hemostasis process promoting by swim bladder. SIGNIFICANCE: Serum proteomics after swim bladder gavage described differentially enriched proteins related to hemostasis, and enriched pathways were validated. This study revealed the possible pathways involved in the hemostatic effect of swim bladder, which may provide a new effector target for the development of new hemostatic drugs.
鱼鳔（干鱼鳔）在中国传统医学中的止血作用是众所周知的。但其作用机制尚不清楚。本研究采用灌胃法给予小鼠中国团头鲂（Nibea coibor）的干鱼鳔。研究了鱼鳔的止血作用，并进行了串联质量标签（TMT）定量蛋白质组学分析，以筛选与小鼠血清止血相关的差异丰度蛋白。结果表明，鱼鳔可显著缩短急性创伤（切除小鼠尾巴）后的出血时间并促进凝血。在对照组和鱼鳔组之间的血清中总共鉴定出 57 个差异表达蛋白，其中 31 个在鱼鳔组中上调，26 个下调。KEGG 通路富集分析进一步表明，中性粒细胞胞外诱捕形成途径受到显著影响。结合 RT-qPCR 验证，我们的研究结果进一步表明，该途径中的 5 种候选蛋白可能参与了鱼鳔灌胃后的止血起始，表明它们在鱼鳔促进止血过程中发挥着重要作用。意义：描述了鱼鳔灌胃后与止血相关的差异富集蛋白及其相关通路，为进一步研究鱼鳔的止血作用机制提供了新的思路。
