College of Acumox and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
Department of Anesthesiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200120, China.
Chin J Integr Med. 2022 Sep;28(9):833-839. doi: 10.1007/s11655-022-2896-1. Epub 2022 Jul 7.
To study the effect of electroacupuncture (EA) on oxaliplatin-induced peripheral neuropathy (OIPN) in rats.
Male Sprague-Dawley rats were equally divided into 3 groups using a random number table: the control group, the OIPN group, and the EA (OIPN + EA) group, with 10 rats in each. The time courses of mechanical, cold sensitivity, and microcirculation blood flow intensity were determined. The morphology of the dorsal root ganglion (DRG) was observed by electron microscopic examination. The protein levels of nuclear factor E2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and the transient receptor potential (TRP) protein family in DRGs were assayed by Western blot.
EA treatment significantly reduced mechanical allodynia and cold allodynia in OIPN rats (P<0.01). Notably, oxaliplatin treatment resulted in impaired microcirculatory blood flow and pathomorphological defects in DRGs (P<0.01). EA treatment increased the microcirculation blood flow and attenuated the pathological changes induced by oxaliplatin (P<0.01). In addition, the expression levels of Nrf2 and HO-1 were down-regulated, and the TRP protein family was over-expressed in the DRGs of OIPN rats (P<0.01). EA increased the expression levels of Nrf2 and HO-1 and decreased the level of TRP protein family in DRG (P<0.05 or P<0.01).
EA may be a potential alternative therapy for OIPN, and its mechanism may be mainly mediated by restoring the Nrf2/HO-1 signaling pathway.
研究电针对奥沙利铂诱导的周围神经病变（OIPN）大鼠的影响。
采用随机数字表法将雄性 Sprague-Dawley 大鼠等分为 3 组：对照组、OIPN 组和电针（OIPN+EA）组，每组 10 只。测定机械、冷敏和微循环血流强度的时程。通过电子显微镜观察背根神经节（DRG）的形态。采用 Western blot 法测定 DRG 中核因子 E2 相关因子 2（Nrf2）、血红素加氧酶-1（HO-1）和瞬时受体电位（TRP）蛋白家族的蛋白水平。
电针治疗可显著减轻 OIPN 大鼠的机械性痛觉过敏和冷感觉过敏（P<0.01）。值得注意的是，奥沙利铂处理导致微循环血流受损和 DRG 的病理形态学缺陷（P<0.01）。电针治疗增加了微循环血流并减轻了奥沙利铂引起的病理变化（P<0.01）。此外，Nrf2 和 HO-1 的表达水平下调，DRG 中 TRP 蛋白家族过度表达（P<0.01）。电针治疗可增加 Nrf2 和 HO-1 的表达水平，降低 DRG 中 TRP 蛋白家族的水平（P<0.05 或 P<0.01）。
电针可能是 OIPN 的一种潜在替代治疗方法，其机制可能主要通过恢复 Nrf2/HO-1 信号通路来介导。
