Libby P R, Porter C W
Biochem Biophys Res Commun. 1987 Apr 14;144(1):528-35. doi: 10.1016/s0006-291x(87)80541-7.
Polyamine oxidase from L1210 cells has been separated into two isozymes, A and B on the basis of chromatography on hydroxyapatite. The two isozymes also show different elution characteristics from MGBG Sepharose and Sephacryl-300. The molecular weights of A and B isozymes are estimated to be 260,000 and 200,000 daltons, respectively. The two isozymes show different kinetic characteristics with spermidine, spermine, N1-acetylspermine, and N1,N12-diacetylspermine.
来自L1210细胞的多胺氧化酶已通过羟基磷灰石柱色谱法分离为两种同工酶A和B。这两种同工酶在MGBG琼脂糖凝胶和Sephacryl - 300上也表现出不同的洗脱特性。A和B同工酶的分子量分别估计为260,000和200,000道尔顿。这两种同工酶对亚精胺、精胺、N1 - 乙酰精胺和N1,N12 - 二乙酰精胺表现出不同的动力学特性。
