Connolly D T, Stoddard B L, Harakas N K, Feder J
Biochem Biophys Res Commun. 1987 Apr 29;144(2):705-12. doi: 10.1016/s0006-291x(87)80022-0.
Human fibroblasts were found to produce a potent mitogen and chemoattractant for fetal bovine aortic endothelial cells. Homogenates from AG1523 and AG1518 foreskin, CCD18Lu lung, and CCD18Co colon fibroblasts produced half-maximal stimulation of endothelial cell growth at concentrations of 1-7 micrograms/ml. The factor was purified from large-scale cultures of the CCD18Co fibroblasts using cation exchange chromatography and heparin-Sepharose chromatography. Such preparations were mitogenic for endothelial cells in vitro at concentrations of about 5-10 ng/ml, and promoted chemotaxis at 0.1-1 ng/ml. Heparinase treatment of the cells prevented the chemotactic response. These properties suggest that the factor may be related to fibroblast growth factor.
研究发现，人成纤维细胞能产生一种对胎牛主动脉内皮细胞具有强大作用的促有丝分裂剂和趋化因子。AG1523和AG1518包皮、CCD18Lu肺以及CCD18Co结肠成纤维细胞的匀浆在浓度为1 - 7微克/毫升时，对内皮细胞生长产生半数最大刺激。该因子通过阳离子交换色谱法和肝素 - 琼脂糖色谱法从CCD18Co成纤维细胞的大规模培养物中纯化得到。此类制剂在体外对内皮细胞具有促有丝分裂作用，浓度约为5 - 10纳克/毫升，并在0.1 - 1纳克/毫升时促进趋化作用。用肝素酶处理细胞可阻止趋化反应。这些特性表明该因子可能与成纤维细胞生长因子有关。
