Centro Fecondazione Assistita (CFA), Via Tasso 480, 80123Naples, Italy.
Zygote. 2022 Oct;30(5):664-673. doi: 10.1017/S0967199422000132. Epub 2022 Jul 8.
The integrity of chromatin in the spermatozoon is essential for reproductive outcome. The aim of this study was to evaluate the most effective and cost-effective method to reduce the percentage of spermatozoa with defects in chromatin decondensation for use in assisted reproductive technologies (ART) procedures. Sperm samples from 15 sub-fertile males were examined at CFA Naples to determine the sperm decondensation index (SDI), using the aniline blue test, before and after preparation, comparing density gradients with two different swim-up approaches. All three techniques led to a reduction in decondensed spermatozoa with no statistical difference ( > 0.05) between the control and the treated sperm. In contrast, we found a highly significant decrease in SDI ( < 0.01) after the two swim-up methods in all the samples, confirming the efficacy of these methods in lowering the percentage of chromatin compaction damage. There was no statistical difference between the two swim-up methods, however swim-up from the pellet led to improved count, motility and the percentage of normal condensed spermatozoa. We suggest that swim-up from the pellet be used in ART on sub-fertile males, both to reduce cell stress by multiple centrifugation and improve the recovery rate of mature spermatozoa.
精子中染色质的完整性对于生殖结果至关重要。本研究旨在评估最有效和最具成本效益的方法，以降低用于辅助生殖技术（ART）程序的染色质解凝聚缺陷精子的百分比。在 CFA Naples，对 15 名亚生育男性的精子样本进行了检查，以使用苯胺蓝试验确定精子解凝聚指数（SDI），并在准备前后进行了比较，比较了两种不同的泳动方法的密度梯度。所有三种技术都导致了解凝聚精子数量的减少，但对照组和处理组之间没有统计学差异（>0.05）。相比之下，我们发现两种泳动方法在所有样本中都导致了 SDI 显著降低（<0.01），证实了这些方法在降低染色质凝聚损伤百分比方面的有效性。然而，两种泳动方法之间没有统计学差异，但从沉淀中泳动导致计数、活力和正常浓缩精子的百分比得到改善。我们建议在亚生育男性的 ART 中使用沉淀泳动，以减少多次离心造成的细胞应激，并提高成熟精子的回收率。
