不同鳞翅目物种保幼激素酯酶的免疫化学特性
Immunochemical characterization of juvenile hormone esterase from different species of Lepidoptera.
作者信息
Wozniak M, Jones D
出版信息
Biochem Biophys Res Commun. 1987 May 14;144(3):1281-6. doi: 10.1016/0006-291x(87)91449-5.
Cross-immunoreactivity of juvenile hormone esterase (JHE) from different species was tested using anti-JHE (Trichoplusia ni) (Noctuidae) polyclonal antibody. Partial cross-reactivity was observed between JHE from Hyphantria cunea, Isia isabella (Arctuidae) and Spodoptera exigua (Noctuidae) in immunoblot analysis. Soluble antigen-antibody complex formation was observed between anti-JHE (T. ni) and antigen(s) from Heliothis virescens (Noctuidae) during immunotitration of antigen(s). Using an ELISA method the highest cross-reactivity was observed for both species from Arctuidae and lower cross-reactivity for antigen(s) from H. virescens.
使用抗（粉纹夜蛾）（夜蛾科）保幼激素酯酶（JHE）多克隆抗体，对不同物种的保幼激素酯酶（JHE）的交叉免疫反应性进行了检测。在免疫印迹分析中，观察到来自美国白蛾、桦尺蛾（灯蛾科）和甜菜夜蛾（夜蛾科）的JHE之间存在部分交叉反应性。在对抗抗原进行免疫滴定期间，观察到抗（粉纹夜蛾）JHE与来自烟芽夜蛾（夜蛾科）的抗原之间形成了可溶性抗原-抗体复合物。使用酶联免疫吸附测定（ELISA）方法，观察到灯蛾科的两个物种的交叉反应性最高，而来自烟芽夜蛾的抗原的交叉反应性较低。
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