Joos L, Gicquaud C
Biochem Cell Biol. 1987 Mar;65(3):261-70. doi: 10.1139/o87-034.
We have developed a new technique for the permeabilization of the membrane of Acanthamoeba castellanii. This technique involves the use of digitonin which alters neither the morphology nor the motility of the cell, but favours the penetration of phalloidin and viroisin. Treatment of permeabilized cells with phalloidin or viroisin induces, in the cortex of the cell, an intensive proliferation of filaments which have been identified as actin. This cortical filamentous layer detaches from the membrane and slowly contracts, acting as a fine mesh sieve which concentrates the organelles in the middle of the cell, causing therefore the formation of a central granuloplasm and a cortical hyaloplasm. During this process, cell motility is irreversibly lost. The results indicate that extensive proliferation and reorganization of actin filaments cannot support cell motility and they are discussed in terms of a general understanding of amoeboid movement.
我们开发了一种用于透化卡氏棘阿米巴细胞膜的新技术。该技术涉及使用洋地黄皂苷，它既不改变细胞的形态也不改变其运动性，但有利于鬼笔环肽和病毒菌素的穿透。用鬼笔环肽或病毒菌素处理透化细胞会在细胞皮层中诱导细丝大量增殖，这些细丝已被鉴定为肌动蛋白。这个皮层丝状层从膜上分离并缓慢收缩，起到一个精细的筛网作用，将细胞器集中在细胞中部，从而导致形成中央颗粒质和皮层透明质。在此过程中，细胞运动性不可逆转地丧失。结果表明，肌动蛋白丝的大量增殖和重组无法支持细胞运动，我们将根据对变形虫运动的一般理解来讨论这些结果。
